Calorimetric and spectroscopic investigation of the helix-to-coil transition of the self-complementary deoxyribonucleotide ATGCAT

Luis A. Marky, Lita Canuel, Roger A. Jones, Kenneth J. Breslauer

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Differential scanning calorimetry and temperature-dependent uv spectroscopy are used to thermodynamically characterize the double-strand to single-strand transition of the self-complementary deoxyribo-oligonucleotide ATGCAT. The calorimetric experiments provide a value of 33.6 kcal (mol of double strand)-1 for the transition between 10 and 90° C. In conjunction with available temperature-dependent nmr data (which reveals terminal base pair fraying), we attempt to define specifically those interactions to which the calorimetrically measured enthalpy change refers. Values of ΔHV.H. (van 't Hoff enthalpy change) are derived from the spectroscopic and calorimetric data and compared with the ΔH obtained directly from the calorimetric experiment. This comparison reveals that the part of the thermally-induced transition that occurs between 10 and 90°C is well represented by a two-state process. It is noted that in assessing the applicability of the two-state model it is best to compare the ΔHcal. with ΔHV.H. obtained from the calorimetric rather than the spectroscopic data.

Original languageEnglish (US)
Pages (from-to)141-149
Number of pages9
JournalBiophysical Chemistry
Volume13
Issue number2
DOIs
StatePublished - Apr 1981
Externally publishedYes

Fingerprint

Deoxyribonucleotides
strands
helices
Enthalpy
coils
Temperature
Differential Scanning Calorimetry
enthalpy
Oligonucleotides
Base Pairing
Differential scanning calorimetry
Spectrum Analysis
oligonucleotides
Experiments
Spectroscopy
trucks
heat measurement
scanning
temperature
spectroscopy

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Organic Chemistry

Cite this

Calorimetric and spectroscopic investigation of the helix-to-coil transition of the self-complementary deoxyribonucleotide ATGCAT. / Marky, Luis A.; Canuel, Lita; Jones, Roger A.; Breslauer, Kenneth J.

In: Biophysical Chemistry, Vol. 13, No. 2, 04.1981, p. 141-149.

Research output: Contribution to journalArticle

@article{f48d98f1044f4a4182d6f238d28e81c2,
title = "Calorimetric and spectroscopic investigation of the helix-to-coil transition of the self-complementary deoxyribonucleotide ATGCAT",
abstract = "Differential scanning calorimetry and temperature-dependent uv spectroscopy are used to thermodynamically characterize the double-strand to single-strand transition of the self-complementary deoxyribo-oligonucleotide ATGCAT. The calorimetric experiments provide a value of 33.6 kcal (mol of double strand)-1 for the transition between 10 and 90° C. In conjunction with available temperature-dependent nmr data (which reveals terminal base pair fraying), we attempt to define specifically those interactions to which the calorimetrically measured enthalpy change refers. Values of ΔHV.H. (van 't Hoff enthalpy change) are derived from the spectroscopic and calorimetric data and compared with the ΔH obtained directly from the calorimetric experiment. This comparison reveals that the part of the thermally-induced transition that occurs between 10 and 90°C is well represented by a two-state process. It is noted that in assessing the applicability of the two-state model it is best to compare the ΔHcal. with ΔHV.H. obtained from the calorimetric rather than the spectroscopic data.",
author = "Marky, {Luis A.} and Lita Canuel and Jones, {Roger A.} and Breslauer, {Kenneth J.}",
year = "1981",
month = "4",
doi = "10.1016/0301-4622(81)80013-0",
language = "English (US)",
volume = "13",
pages = "141--149",
journal = "Biophysical Chemistry",
issn = "0301-4622",
publisher = "Elsevier",
number = "2",

}

TY - JOUR

T1 - Calorimetric and spectroscopic investigation of the helix-to-coil transition of the self-complementary deoxyribonucleotide ATGCAT

AU - Marky, Luis A.

AU - Canuel, Lita

AU - Jones, Roger A.

AU - Breslauer, Kenneth J.

PY - 1981/4

Y1 - 1981/4

N2 - Differential scanning calorimetry and temperature-dependent uv spectroscopy are used to thermodynamically characterize the double-strand to single-strand transition of the self-complementary deoxyribo-oligonucleotide ATGCAT. The calorimetric experiments provide a value of 33.6 kcal (mol of double strand)-1 for the transition between 10 and 90° C. In conjunction with available temperature-dependent nmr data (which reveals terminal base pair fraying), we attempt to define specifically those interactions to which the calorimetrically measured enthalpy change refers. Values of ΔHV.H. (van 't Hoff enthalpy change) are derived from the spectroscopic and calorimetric data and compared with the ΔH obtained directly from the calorimetric experiment. This comparison reveals that the part of the thermally-induced transition that occurs between 10 and 90°C is well represented by a two-state process. It is noted that in assessing the applicability of the two-state model it is best to compare the ΔHcal. with ΔHV.H. obtained from the calorimetric rather than the spectroscopic data.

AB - Differential scanning calorimetry and temperature-dependent uv spectroscopy are used to thermodynamically characterize the double-strand to single-strand transition of the self-complementary deoxyribo-oligonucleotide ATGCAT. The calorimetric experiments provide a value of 33.6 kcal (mol of double strand)-1 for the transition between 10 and 90° C. In conjunction with available temperature-dependent nmr data (which reveals terminal base pair fraying), we attempt to define specifically those interactions to which the calorimetrically measured enthalpy change refers. Values of ΔHV.H. (van 't Hoff enthalpy change) are derived from the spectroscopic and calorimetric data and compared with the ΔH obtained directly from the calorimetric experiment. This comparison reveals that the part of the thermally-induced transition that occurs between 10 and 90°C is well represented by a two-state process. It is noted that in assessing the applicability of the two-state model it is best to compare the ΔHcal. with ΔHV.H. obtained from the calorimetric rather than the spectroscopic data.

UR - http://www.scopus.com/inward/record.url?scp=0019551185&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019551185&partnerID=8YFLogxK

U2 - 10.1016/0301-4622(81)80013-0

DO - 10.1016/0301-4622(81)80013-0

M3 - Article

C2 - 7260332

AN - SCOPUS:0019551185

VL - 13

SP - 141

EP - 149

JO - Biophysical Chemistry

JF - Biophysical Chemistry

SN - 0301-4622

IS - 2

ER -