Calmodulin activity in yeast and mycelial phases of Ceratocystis ulmi

Ganapathy Muthukumar, Maureen T. Luby, Kenneth W. Nickerson

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The yeast and mycelial phases of Ceratocystis ulmi contained roughly equivalent levels of calmodulin activity as determined by their ability to stimulate calmodulin-deficient bovine brain cAMP phosphodiesterase. This stimulation was calcium-dependent and could be inhibited by either dibucaine or trifluoperazine. Also, the concentration of dibucaine necessary to achieve the mycelium-to-yeast morphological conversion was found to be 3-fold greater in the presence of exogenous calcium. A model is presented in which only 30% of the cellular calmodulin need be complexed with calcium ions for mycelial development.

Original languageEnglish (US)
Pages (from-to)313-316
Number of pages4
JournalFEMS Microbiology Letters
Volume37
Issue number3
DOIs
StatePublished - Dec 1986

Fingerprint

Ulmus
Calmodulin
Dibucaine
Yeasts
Calcium
Trifluoperazine
Mycelium
Phosphoric Diester Hydrolases
Ions
Brain

Keywords

  • Ceratocystis ulmi
  • calcium
  • calmodulin
  • cyclic nucleotide phosphodiesterase
  • dimorphism
  • trifluoperazine

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Genetics

Cite this

Calmodulin activity in yeast and mycelial phases of Ceratocystis ulmi. / Muthukumar, Ganapathy; Luby, Maureen T.; Nickerson, Kenneth W.

In: FEMS Microbiology Letters, Vol. 37, No. 3, 12.1986, p. 313-316.

Research output: Contribution to journalArticle

Muthukumar, Ganapathy ; Luby, Maureen T. ; Nickerson, Kenneth W. / Calmodulin activity in yeast and mycelial phases of Ceratocystis ulmi. In: FEMS Microbiology Letters. 1986 ; Vol. 37, No. 3. pp. 313-316.
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