Calcium-permeable AMPA receptors containing Q/R-unedited GluR2 direct human neural progenitor cell differentiation to neurons

Nicholas P. Whitney, Hui Peng, Nathan B. Erdmann, Changhai Tian, Daniel T Monaghan, Jialin C Zheng

Research output: Contribution to journalArticle

63 Citations (Scopus)

Abstract

We identify calcium-permeable α-amino-3-hydroxy-5-methyl-4- isoxazolepropionic acid (AMPA) receptors on human neural progenitor cells (NPCs) and present a physiological role in neurogenesis. RNA editing of the GluR2 subunit at the Q/R site is responsible for making most AMPA receptors impermeable to calcium. Because a single-point mutation could eliminate the need for editing at the Q/R site and Q/R-unedited GluR2 exists during embryogenesis, the Q/R-unedited GluR2 subunit presumably has some important actions early in development. Using calcium imaging, we found that NPCs contain calcium-permeable AMPA receptors, whereas NPCs differentiated to neurons and astrocytes express calcium-impermeable AMPA receptors. We utilized reverse-transcription polymerase chain reaction and BbvI digestion to demonstrate that NPCs contain Q/R-unedited GluR2, and differentiated cells contain Q/R-edited GluR2 subunits. This is consistent with the observation that the nuclear enzyme responsible for Q/R-editing, adenosine deaminase (ADAR2), is increased during differentiation. Activation of calcium-permeable AMPA receptors induces NPCs to differentiate to the neuronal lineage and increases dendritic arbor formation in NPCs differentiated to neurons. AMPA-induced differentiation of NPCs to neurons is abrogated by overexpression of ADAR2 in NPCs. This elucidates the role of AMPA receptors as inductors of neurogenesis and provides a possible explanation for why the Q/R editing process exists.

Original languageEnglish (US)
Pages (from-to)2888-2900
Number of pages13
JournalFASEB Journal
Volume22
Issue number8
DOIs
StatePublished - Aug 1 2008

Fingerprint

AMPA Receptors
cell differentiation
Neurons
stem cells
Cell Differentiation
Stem Cells
neurons
Calcium
calcium
receptors
neurogenesis
Neurogenesis
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Adenosine Deaminase
Polymerase chain reaction
Transcription
1,4-alpha-glucan branching enzyme
RNA Editing
adenosine deaminase
RNA editing

Keywords

  • ADAR2
  • Dendritic arborization
  • Glutamate
  • Neurogenesis
  • Q/R editing

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Calcium-permeable AMPA receptors containing Q/R-unedited GluR2 direct human neural progenitor cell differentiation to neurons. / Whitney, Nicholas P.; Peng, Hui; Erdmann, Nathan B.; Tian, Changhai; Monaghan, Daniel T; Zheng, Jialin C.

In: FASEB Journal, Vol. 22, No. 8, 01.08.2008, p. 2888-2900.

Research output: Contribution to journalArticle

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