Calcined Eggshell Waste for Mitigating Soil Antibiotic-Resistant Bacteria/Antibiotic Resistance Gene Dissemination and Accumulation in Bell Pepper

Mao Ye, Mingming Sun, Yanfang Feng, Xu Li, Arthur P. Schwab, Jinzhong Wan, Manqiang Liu, Da Tian, Kuan Liu, Jun Wu, Xin Jiang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The combined accumulation of antibiotics, heavy metals, antibiotic-resistant bacteria (ARB)/antibiotic resistance genes (ARGs) in vegetables has become a new threat to human health. This is the first study to investigate the feasibility of calcined eggshells modified by aluminum sulfate as novel agricultural wastes to impede mixed contaminants from transferring to bell pepper (Capsicum annuum L.). In this work, calcined eggshell amendment mitigated mixed pollutant accumulation in bell pepper significantly, enhanced the dissipation of soil tetracycline, sulfadiazine, roxithromycin, and chloramphenicol, decreased the water-soluble fractions of antibiotics, and declined the diversity of ARB/ARGs inside the vegetable. Moreover, quantitative polymerase chain reaction analysis detected that ARG levels in the bell pepper fruits significantly decreased to 10-10 copies/16S copies, indicating limited risk of ARGs transferring along the food chain. Furthermore, the restoration of soil microbial biological function suggests that calcined eggshell is an environmentally friendly amendment to control the dissemination of soil ARB/ARGs in the soil-vegetable system.

Original languageEnglish (US)
Pages (from-to)5446-5453
Number of pages8
JournalJournal of Agricultural and Food Chemistry
Volume64
Issue number27
DOIs
StatePublished - Jul 13 2016

Fingerprint

Egg Shell
Capsicum
sweet peppers
Microbial Drug Resistance
egg shell
antibiotic resistance
Bacteria
Soil
Genes
antibiotics
Anti-Bacterial Agents
Soils
Vegetables
bacteria
vegetables
soil
genes
roxithromycin
Roxithromycin
aluminum sulfate

Keywords

  • antibiotic resistance genes
  • antibiotic-resistant bacteria
  • bell pepper cultivation
  • calcined eggshells
  • impedance effect

ASJC Scopus subject areas

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

Calcined Eggshell Waste for Mitigating Soil Antibiotic-Resistant Bacteria/Antibiotic Resistance Gene Dissemination and Accumulation in Bell Pepper. / Ye, Mao; Sun, Mingming; Feng, Yanfang; Li, Xu; Schwab, Arthur P.; Wan, Jinzhong; Liu, Manqiang; Tian, Da; Liu, Kuan; Wu, Jun; Jiang, Xin.

In: Journal of Agricultural and Food Chemistry, Vol. 64, No. 27, 13.07.2016, p. 5446-5453.

Research output: Contribution to journalArticle

Ye, Mao ; Sun, Mingming ; Feng, Yanfang ; Li, Xu ; Schwab, Arthur P. ; Wan, Jinzhong ; Liu, Manqiang ; Tian, Da ; Liu, Kuan ; Wu, Jun ; Jiang, Xin. / Calcined Eggshell Waste for Mitigating Soil Antibiotic-Resistant Bacteria/Antibiotic Resistance Gene Dissemination and Accumulation in Bell Pepper. In: Journal of Agricultural and Food Chemistry. 2016 ; Vol. 64, No. 27. pp. 5446-5453.
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