Cadmium increases tissue factor (coagulation factor III) activity by facilitating its reassociation with lipids

Steven D Carson, William H. Konigsberg

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

The coagulant activity of partially purified and delipidated tissue factor (TF) (coagulation Factor III) has previously been recovered by dialysis of the apoprotein after addition of mixed brain lipids and deoxycholate. Inclusion of cadmium chloride in the relipidation mixture greatly increases the recovered activity of highly purified TF from human placenta by promoting incorporation of TF into phospholipid vesicles; TF that had not been incorporated into vesicles showed no coagulant activity. Thus, TF must be present in a lipid bilayer for expression of coagulant activity. In vitro, cadmium induces fusion of lipid vesicles and may contribute to the incorporation of proteins in membranes.

Original languageEnglish (US)
Pages (from-to)307-309
Number of pages3
JournalScience
Volume208
Issue number4441
DOIs
StatePublished - Jan 1 1980

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Thromboplastin
Cadmium
Lipids
Coagulants
Cadmium Chloride
Apoproteins
Deoxycholic Acid
Lipid Bilayers
Placenta
Dialysis
Phospholipids
Membrane Proteins
Brain

ASJC Scopus subject areas

  • General

Cite this

Cadmium increases tissue factor (coagulation factor III) activity by facilitating its reassociation with lipids. / Carson, Steven D; Konigsberg, William H.

In: Science, Vol. 208, No. 4441, 01.01.1980, p. 307-309.

Research output: Contribution to journalArticle

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