Cadmium causes vesicle leakage under conditions which favor reconstitution of tissue factor-vesicle complexes

Research output: Contribution to journalArticle

Abstract

Cadmium has a singular ability to promote the functional reconstitution of tissue factor (coagulation factor III) apoprotein with vesicles formed from mixed bovine brain lipids. Cadmium causes rapid release of carboxyfluorescein from lipid vesicles which chromatograph in the included volume of Bio Gel A5m, and also promotes reconstitution of tissue factor with these vesicles. With larger vesicles, which are excluded on Bio Gel A5m, cadmium produces only low levels of reconstitution and leakage. Calcium has been shown to promote only low levels of tissue factor-vesicle reconstitution, and produces only low levels of carboxyfluorescein leakage when compared to cadmium. These results demonstrate that cadmium and calcium interact differently with the small mixed lipid vesicles, and suggest that the cadmium-promoted reconstitution of tissue factor vesicle-complexes accompanies cadmium-induced alterations in vesicle structure. The results also show that cadmium affects large and small vesicles differently.

Original languageEnglish (US)
Pages (from-to)123-127
Number of pages5
JournalThe Journal of Membrane Biology
Volume75
Issue number2
DOIs
StatePublished - Jul 1 1983

Fingerprint

Thromboplastin
Cadmium
Lipids
Gels
Calcium
Apoproteins
Brain

Keywords

  • cadmium
  • coagulation factor III
  • membrane reconstitution
  • tissue factor
  • vesicles

ASJC Scopus subject areas

  • Physiology
  • Cell Biology
  • Biophysics

Cite this

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abstract = "Cadmium has a singular ability to promote the functional reconstitution of tissue factor (coagulation factor III) apoprotein with vesicles formed from mixed bovine brain lipids. Cadmium causes rapid release of carboxyfluorescein from lipid vesicles which chromatograph in the included volume of Bio Gel A5m, and also promotes reconstitution of tissue factor with these vesicles. With larger vesicles, which are excluded on Bio Gel A5m, cadmium produces only low levels of reconstitution and leakage. Calcium has been shown to promote only low levels of tissue factor-vesicle reconstitution, and produces only low levels of carboxyfluorescein leakage when compared to cadmium. These results demonstrate that cadmium and calcium interact differently with the small mixed lipid vesicles, and suggest that the cadmium-promoted reconstitution of tissue factor vesicle-complexes accompanies cadmium-induced alterations in vesicle structure. The results also show that cadmium affects large and small vesicles differently.",
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