Cadaverine and aminoguanidine potentiate the uptake of histamine in vitro in perfused intestinal segments of rats

D. E. Lyons, J. T. Beery, S. A. Lyons, S. L. Taylor

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Examination of the serosal fluid following in vitro luminal perfusion of rat intestinal segments with 1 mg/ml [3H]histamine for 2 hr showed that histamine constituted only 22.1% of the total serosal radioactivity. The remainder of the radioactivity was comprised of histamine metabolites. When equimolar amounts of either aminoguanidine and cadaverine were added to the luminal perfusate, the percentage of the serosal radioactivity as histamine increased to 67.0 and 60.4%, respectively. However, when equal amounts of histamine and anserine were added to the luminal perfusate, only 30.6% of the 3H translocated within 2 hr was [3H]histamine. In all cases, the gross translocation rate based on the percentage of total serosal radioactivity for total radioisotope ([3H]histamine plus [3H]histamine metabolites) was unchanged by the addition of these substances to the luminal perfusate. The results indicate that the potentiation of histamine toxicity by putrefactive amines, such as cadaverine, results from the inhibition of histamine metabolism which leads to increased uptake of unmetabolized histamine. The results do not support the hypothesis that potentiation occurs via an overall increase in the absorption of histamine and its metabolites due to some disruption in the barrier function of the intestine.

Original languageEnglish (US)
Pages (from-to)445-458
Number of pages14
JournalToxicology and Applied Pharmacology
Volume70
Issue number3
DOIs
StatePublished - Sep 30 1983

Fingerprint

Cadaverine
Histamine
Rats
Radioactivity
Metabolites
pimagedine
In Vitro Techniques
Anserine
Enzyme inhibition
Metabolism
Radioisotopes
Intestines
Amines
Toxicity

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology

Cite this

Cadaverine and aminoguanidine potentiate the uptake of histamine in vitro in perfused intestinal segments of rats. / Lyons, D. E.; Beery, J. T.; Lyons, S. A.; Taylor, S. L.

In: Toxicology and Applied Pharmacology, Vol. 70, No. 3, 30.09.1983, p. 445-458.

Research output: Contribution to journalArticle

@article{32a2152bba3e489eab6d546b68ae5492,
title = "Cadaverine and aminoguanidine potentiate the uptake of histamine in vitro in perfused intestinal segments of rats",
abstract = "Examination of the serosal fluid following in vitro luminal perfusion of rat intestinal segments with 1 mg/ml [3H]histamine for 2 hr showed that histamine constituted only 22.1{\%} of the total serosal radioactivity. The remainder of the radioactivity was comprised of histamine metabolites. When equimolar amounts of either aminoguanidine and cadaverine were added to the luminal perfusate, the percentage of the serosal radioactivity as histamine increased to 67.0 and 60.4{\%}, respectively. However, when equal amounts of histamine and anserine were added to the luminal perfusate, only 30.6{\%} of the 3H translocated within 2 hr was [3H]histamine. In all cases, the gross translocation rate based on the percentage of total serosal radioactivity for total radioisotope ([3H]histamine plus [3H]histamine metabolites) was unchanged by the addition of these substances to the luminal perfusate. The results indicate that the potentiation of histamine toxicity by putrefactive amines, such as cadaverine, results from the inhibition of histamine metabolism which leads to increased uptake of unmetabolized histamine. The results do not support the hypothesis that potentiation occurs via an overall increase in the absorption of histamine and its metabolites due to some disruption in the barrier function of the intestine.",
author = "Lyons, {D. E.} and Beery, {J. T.} and Lyons, {S. A.} and Taylor, {S. L.}",
year = "1983",
month = "9",
day = "30",
doi = "10.1016/0041-008X(83)90162-X",
language = "English (US)",
volume = "70",
pages = "445--458",
journal = "Toxicology and Applied Pharmacology",
issn = "0041-008X",
publisher = "Academic Press Inc.",
number = "3",

}

TY - JOUR

T1 - Cadaverine and aminoguanidine potentiate the uptake of histamine in vitro in perfused intestinal segments of rats

AU - Lyons, D. E.

AU - Beery, J. T.

AU - Lyons, S. A.

AU - Taylor, S. L.

PY - 1983/9/30

Y1 - 1983/9/30

N2 - Examination of the serosal fluid following in vitro luminal perfusion of rat intestinal segments with 1 mg/ml [3H]histamine for 2 hr showed that histamine constituted only 22.1% of the total serosal radioactivity. The remainder of the radioactivity was comprised of histamine metabolites. When equimolar amounts of either aminoguanidine and cadaverine were added to the luminal perfusate, the percentage of the serosal radioactivity as histamine increased to 67.0 and 60.4%, respectively. However, when equal amounts of histamine and anserine were added to the luminal perfusate, only 30.6% of the 3H translocated within 2 hr was [3H]histamine. In all cases, the gross translocation rate based on the percentage of total serosal radioactivity for total radioisotope ([3H]histamine plus [3H]histamine metabolites) was unchanged by the addition of these substances to the luminal perfusate. The results indicate that the potentiation of histamine toxicity by putrefactive amines, such as cadaverine, results from the inhibition of histamine metabolism which leads to increased uptake of unmetabolized histamine. The results do not support the hypothesis that potentiation occurs via an overall increase in the absorption of histamine and its metabolites due to some disruption in the barrier function of the intestine.

AB - Examination of the serosal fluid following in vitro luminal perfusion of rat intestinal segments with 1 mg/ml [3H]histamine for 2 hr showed that histamine constituted only 22.1% of the total serosal radioactivity. The remainder of the radioactivity was comprised of histamine metabolites. When equimolar amounts of either aminoguanidine and cadaverine were added to the luminal perfusate, the percentage of the serosal radioactivity as histamine increased to 67.0 and 60.4%, respectively. However, when equal amounts of histamine and anserine were added to the luminal perfusate, only 30.6% of the 3H translocated within 2 hr was [3H]histamine. In all cases, the gross translocation rate based on the percentage of total serosal radioactivity for total radioisotope ([3H]histamine plus [3H]histamine metabolites) was unchanged by the addition of these substances to the luminal perfusate. The results indicate that the potentiation of histamine toxicity by putrefactive amines, such as cadaverine, results from the inhibition of histamine metabolism which leads to increased uptake of unmetabolized histamine. The results do not support the hypothesis that potentiation occurs via an overall increase in the absorption of histamine and its metabolites due to some disruption in the barrier function of the intestine.

UR - http://www.scopus.com/inward/record.url?scp=0021084621&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021084621&partnerID=8YFLogxK

U2 - 10.1016/0041-008X(83)90162-X

DO - 10.1016/0041-008X(83)90162-X

M3 - Article

C2 - 6636174

AN - SCOPUS:0021084621

VL - 70

SP - 445

EP - 458

JO - Toxicology and Applied Pharmacology

JF - Toxicology and Applied Pharmacology

SN - 0041-008X

IS - 3

ER -