CA Runs Increase DNA Flexibility in the Complex of λ Cro Protein with the OR3 Site

Yuri L. Lyubchenko, Lyuda S. Shlyakhtenko, Rodney E. Harrington, Rodney E. Harrington

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

The alternating pyrimidine–purine elements CA, CAC, and CACA are anisotropically flexible, as deduced from gel circularization assays on point mutations and single-base mismatches in the OR3 site of λ phage alone and in the specific complex with the Cro protein. These sequences evidently promote DNA bending in the specific binding region of the complex and may also facilitate overwinding in the central nonbinding region. Effects for CACA are exceptionally large and suggest that an alternative DNA structure may occur in this element.

Original languageEnglish (US)
Pages (from-to)4121-4127
Number of pages7
JournalBiochemistry
Volume32
Issue number15
DOIs
StatePublished - Apr 1 1993

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Bacteriophages
DNA
Point Mutation
Assays
Proteins
Gels
N(4)-chloroacetylcytosine arabinoside

ASJC Scopus subject areas

  • Biochemistry

Cite this

CA Runs Increase DNA Flexibility in the Complex of λ Cro Protein with the OR3 Site. / Lyubchenko, Yuri L.; Shlyakhtenko, Lyuda S.; Harrington, Rodney E.; Harrington, Rodney E.

In: Biochemistry, Vol. 32, No. 15, 01.04.1993, p. 4121-4127.

Research output: Contribution to journalArticle

Lyubchenko, YL, Shlyakhtenko, LS, Harrington, RE & Harrington, RE 1993, 'CA Runs Increase DNA Flexibility in the Complex of λ Cro Protein with the OR3 Site', Biochemistry, vol. 32, no. 15, pp. 4121-4127. https://doi.org/10.1021/bi00066a038
Lyubchenko, Yuri L. ; Shlyakhtenko, Lyuda S. ; Harrington, Rodney E. ; Harrington, Rodney E. / CA Runs Increase DNA Flexibility in the Complex of λ Cro Protein with the OR3 Site. In: Biochemistry. 1993 ; Vol. 32, No. 15. pp. 4121-4127.
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