C3 synthesis by A549 alveolar epithelial cells is increased by interferon-γ and dexamethasone

Laura Diane Bilek, L. Sun, M. P. Leuschen, T. L. Zach

Research output: Contribution to journalArticle

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Abstract

The third component of complement, C3, is produced in the lung by several cell types including alveolar epithelial cells. Since interferon-γ (IFN-γ) and dexamethasone regulate C3 gene expression in non-pulmonary cells, and because IFN-γ and dexamethasone interact to regulate the functional activity of alveolar epithelial cells, we investigated the effects of IFN-γ and dexamethasone on C3 production by A549 human alveolar epithelial cells. Treatment of A549 cells with IFN-γ alone increased C3 production in a time- and dose-dependent manner. Maximal increase in C3 production occurred after stimulation of A549 cells with 500 IU/ml IFN-γ for 3 days and was 3.4-fold greater than control. Dexamethasone (0.1 μM) stimulation of A549 cells increased C3 production 6.7-fold over controls on day 3. Treatment of A549 cells with IFN-γ plus dexamethasone resulted in an 11- to 13-fold increase in C3 synthesis. C3 mRNA levels were increased in A549 cells treated with IFN-γ and dexamethasone individually and in combination suggesting that IFN-γ and dexamethasone increase C3 synthesis by a pre-translational mechanism. IFN-γ and dexamethasone did not alter the two chain structure of the C3 molecule produced by A549 cells, as assessed by Western blotting. We speculate that IFN-γ and glucocorticoids may be important in the local regulation of C3 synthesis in the lung.

Original languageEnglish (US)
Pages (from-to)236-240
Number of pages5
JournalImmunology
Volume79
Issue number2
StatePublished - Jan 1 1993

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Alveolar Epithelial Cells
Interferons
Dexamethasone
A549 Cells
Lung
Complement C3
Glucocorticoids
Western Blotting

ASJC Scopus subject areas

  • Immunology

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C3 synthesis by A549 alveolar epithelial cells is increased by interferon-γ and dexamethasone. / Bilek, Laura Diane; Sun, L.; Leuschen, M. P.; Zach, T. L.

In: Immunology, Vol. 79, No. 2, 01.01.1993, p. 236-240.

Research output: Contribution to journalArticle

Bilek, Laura Diane ; Sun, L. ; Leuschen, M. P. ; Zach, T. L. / C3 synthesis by A549 alveolar epithelial cells is increased by interferon-γ and dexamethasone. In: Immunology. 1993 ; Vol. 79, No. 2. pp. 236-240.
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