C1- and R-dependent expression of the maize Bz1 gene requires sequences with homology to mammalian myb and myc binding sites

Bradley A. Roth, Stephen A. Goff, Theodore M. Klein, Michael E Fromm

Research output: Contribution to journalArticle

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Abstract

Tissue-specific expression of the maize anthocyanin Bronze-1 (Bz1) gene is controlled by the products of several regulatory genes. These include C1 or PI and R or B that share homology to the myb proto-oncogenes and myc-like genes respectively. Bz1 expression in embryo tissues is dependent on C1 and an R-sc allele of R. Transient expression from mutated and deleted versions of the Bz1 promoter fused to a luciferase reporter gene was measured in C1, Rscm2 embryos after gene transfer by microprojectiles. This analysis revealed that the sequences between -76 base pairs (bp) and -45 bp and a 9-bp AT-rich block between -88 bp and -80 bp were critical for Bz1 expression The -76 bp to -45 bp region includes two short sequences that are homologous to the consensus binding sites of the myb- and myc-like proteins. Site-specific mutations of these "myb" and "myc" sequences reduced Bz1 expression to 10% and 1% of normal, respectively. Additionally, a trimer of a 38-bp ohgonucleotide containing these myb and myc sites increased the expression of a cauliflower mosaic virus 35S minimal promoter by 26-fold This enhancement was dependent on both C1 and R. Because the sites critical for Bz1 expression are homologous to the myb and myc consensus binding sequences and the C1 and R proteins share homology with the myb and myc products, respectively, we propose that C1 and R interact with the Bz1 promoter at these sites.

Original languageEnglish (US)
Pages (from-to)317-325
Number of pages9
JournalPlant Cell
Volume3
Issue number3
StatePublished - Mar 1 1991

Fingerprint

Bronze
Sequence Homology
Base Pairing
Zea mays
binding sites
Genes
Binding Sites
promoter regions
nucleotide sequences
corn
proto-oncogenes
Cauliflower mosaic virus
luciferase
regulator genes
gene transfer
reporter genes
anthocyanins
genes
sequence analysis
proteins

ASJC Scopus subject areas

  • Plant Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

C1- and R-dependent expression of the maize Bz1 gene requires sequences with homology to mammalian myb and myc binding sites. / Roth, Bradley A.; Goff, Stephen A.; Klein, Theodore M.; Fromm, Michael E.

In: Plant Cell, Vol. 3, No. 3, 01.03.1991, p. 317-325.

Research output: Contribution to journalArticle

Roth, Bradley A. ; Goff, Stephen A. ; Klein, Theodore M. ; Fromm, Michael E. / C1- and R-dependent expression of the maize Bz1 gene requires sequences with homology to mammalian myb and myc binding sites. In: Plant Cell. 1991 ; Vol. 3, No. 3. pp. 317-325.
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