c-Myc promoter activation in medulloblastoma

I. Mei Siu, Ahita Lal, Jill R Blankenship, Naji Aldosari, Gregory J. Riggins

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The c-myc oncogene is commonly activated in medulloblastoma. Genomic amplification is a well-documented cause of c-myc activation but does not account for all cases of c-myc activation. In this study, we sought other means by which c-myc is overexpressed in medulloblastoma. Twelve medulloblastoma or PNET cell lines were screened for c-myc genomic amplification, mRNA levels, and protein levels. Two medulloblastoma lines, D283 Med and D721 Med, were identified that expressed c-myc mRNA and protein at high levels without genomic amplification. The c-myc gene's regulatory sequences were normal in those cell lines. However, specific regions of the promoter, independent of the β-catenin binding sites, were responsible for activation as revealed by promoter assays and site-directed mutagenesis. Transcriptional activation by a β-catenin-independent pathway is therefore a likely mechanism for c-myc overexpression in a subset of medulloblastomas.

Original languageEnglish (US)
Pages (from-to)4773-4776
Number of pages4
JournalCancer Research
Volume63
Issue number16
StatePublished - Aug 15 2003

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Medulloblastoma
Catenins
myc Genes
Proto-Oncogene Proteins c-myc
Primitive Neuroectodermal Tumors
Cell Line
Messenger RNA
Site-Directed Mutagenesis
Genetic Promoter Regions
Transcriptional Activation
Binding Sites
Proteins

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Siu, I. M., Lal, A., Blankenship, J. R., Aldosari, N., & Riggins, G. J. (2003). c-Myc promoter activation in medulloblastoma. Cancer Research, 63(16), 4773-4776.

c-Myc promoter activation in medulloblastoma. / Siu, I. Mei; Lal, Ahita; Blankenship, Jill R; Aldosari, Naji; Riggins, Gregory J.

In: Cancer Research, Vol. 63, No. 16, 15.08.2003, p. 4773-4776.

Research output: Contribution to journalArticle

Siu, IM, Lal, A, Blankenship, JR, Aldosari, N & Riggins, GJ 2003, 'c-Myc promoter activation in medulloblastoma', Cancer Research, vol. 63, no. 16, pp. 4773-4776.
Siu IM, Lal A, Blankenship JR, Aldosari N, Riggins GJ. c-Myc promoter activation in medulloblastoma. Cancer Research. 2003 Aug 15;63(16):4773-4776.
Siu, I. Mei ; Lal, Ahita ; Blankenship, Jill R ; Aldosari, Naji ; Riggins, Gregory J. / c-Myc promoter activation in medulloblastoma. In: Cancer Research. 2003 ; Vol. 63, No. 16. pp. 4773-4776.
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