C-Jun terminal kinases play an important role in regulating embryonic survival and eye development in vertebrates

X. Q. Huang, Z. X. Huang, Z. L. Li, X. W. Chen, X. Li, X. C. Tang, F. Y. Liu, Y. Liu, L. Chen, W. J. Han, L. Liu, L. Li, K. L. Wu, M. X. Wu, X. L. Liu, L. X. Luo, M. B. Yu, Yi Zhi Liu, Shao Jun Liu, David Wan Cheng Li

Research output: Contribution to journalArticle

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Abstract

The c-Jun N-terminal kinases (JNKs) constitute one of the three major types of mitogen-activated protein kinases. Previous studies showed that JNK mediates multiple signaling transduction pathways implicated in cell proliferation, differentiation, inflammation, stress response and apoptosis in mammals. In the present study, we use goldfish as a model system and demonstrate that JNK kinases are necessary to promote embryonic survival and regulate eye development in vertebrates. During goldfish development, JNK1 and JNK2 are expressed at every stage from cleavage to hatching larvae. JNK3 is turned on at the gastrulation stage and then expressed at similar level to that of JNK2. JNK1 activity remains slightly fluctuated during different developmental stages. Inhibition of JNK activity caused massive apoptosis of blastula cells and significant death of goldfish embryos, which are associated with altered expression of the anti-apoptotic regulator, Mcl-1 and the proapoptotic regulator, Bak. These results provide novel information regarding the mechanisms by which JNKs promote embryonic survival. In addition, the embryos that survived inhibition of JNK activity displayed severe phenotype in the eye with clear microphthalmia and lens coloboma. To confirm that the observed phenotype is derived from JNK activity deficiency, we expressed JNK dominant negative mutant (DNM-JNK) in goldfish. Expression of DNM-JNK also caused similar phenotypes with altered expression of pax-6, Sox-2 and β-crystallin. Together, our results demonstrate that JNKs play important roles in promoting survival of vertebrate embryos and regulating development of vertebrate eye.

Original languageEnglish (US)
Pages (from-to)228-237
Number of pages10
JournalCurrent Molecular Medicine
Volume13
Issue number1
DOIs
StatePublished - Jan 1 2013

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Vertebrates
Phosphotransferases
Goldfish
Phenotype
Embryonic Structures
Apoptosis
Naphazoline
Coloboma
Microphthalmos
Blastula
Gastrulation
Crystallins
Mammals
JNK Mitogen-Activated Protein Kinases
Cell proliferation
Mitogen-Activated Protein Kinases
Lenses
Embryonic Development
Larva
Cell Differentiation

Keywords

  • Cataract
  • Cell differentiation
  • Development
  • JNK1
  • JNK2
  • JNK3
  • Lens
  • Retina

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology

Cite this

C-Jun terminal kinases play an important role in regulating embryonic survival and eye development in vertebrates. / Huang, X. Q.; Huang, Z. X.; Li, Z. L.; Chen, X. W.; Li, X.; Tang, X. C.; Liu, F. Y.; Liu, Y.; Chen, L.; Han, W. J.; Liu, L.; Li, L.; Wu, K. L.; Wu, M. X.; Liu, X. L.; Luo, L. X.; Yu, M. B.; Liu, Yi Zhi; Liu, Shao Jun; Li, David Wan Cheng.

In: Current Molecular Medicine, Vol. 13, No. 1, 01.01.2013, p. 228-237.

Research output: Contribution to journalArticle

Huang, XQ, Huang, ZX, Li, ZL, Chen, XW, Li, X, Tang, XC, Liu, FY, Liu, Y, Chen, L, Han, WJ, Liu, L, Li, L, Wu, KL, Wu, MX, Liu, XL, Luo, LX, Yu, MB, Liu, YZ, Liu, SJ & Li, DWC 2013, 'C-Jun terminal kinases play an important role in regulating embryonic survival and eye development in vertebrates', Current Molecular Medicine, vol. 13, no. 1, pp. 228-237. https://doi.org/10.2174/156652413804486278
Huang, X. Q. ; Huang, Z. X. ; Li, Z. L. ; Chen, X. W. ; Li, X. ; Tang, X. C. ; Liu, F. Y. ; Liu, Y. ; Chen, L. ; Han, W. J. ; Liu, L. ; Li, L. ; Wu, K. L. ; Wu, M. X. ; Liu, X. L. ; Luo, L. X. ; Yu, M. B. ; Liu, Yi Zhi ; Liu, Shao Jun ; Li, David Wan Cheng. / C-Jun terminal kinases play an important role in regulating embryonic survival and eye development in vertebrates. In: Current Molecular Medicine. 2013 ; Vol. 13, No. 1. pp. 228-237.
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