Butyrate and deoxycholic acid play common and distinct roles in HCT116 human colon cell proliferation

Huawei Zeng, Kate J. Claycombe, Katie M. Reindl

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Consumption of a high-fat diet causes an increase in bile acid deoxycholic acid (DCA) in colon lumen and colon cancer risk, while butyrate, an intestinal microbiota metabolite of dietary fiber, has been shown to exhibit colon cancer-preventive effects. To distinguish these opposing effects of DCA and butyrate (two major metabolites in colon lumen), we examined the effects of physiologically relevant doses of butyrate (0.5-2 mmol/l) and DCA (0.05-0.3 mmol/l) on colon cell proliferation. We hypothesize that butyrate and DCA each modulates the cell cycle and apoptosis via common and distinct cellular signaling targets. In this study, we demonstrated that both butyrate and DCA inhibited cell proliferation by up to 89% and 92% and increased cell apoptosis rate by up to 3.1- and 4.5-fold, respectively. Cell cycle analyses revealed that butyrate led to an increase in G1 and G2 fractions with a concomitant drop in the S-phase fraction, but DCA induced an increase in only G1 fraction with a concomitant drop in the S-phase fraction when compared with the untreated cells. The examination of early cellular signaling revealed that DCA but not butyrate increased intracellular reactive oxygen species, genomic DNA breakage, the activation of ERK1/2, caspase-3 and PARP. In contrast, DCA decreased activated Rb protein level, and butyrate but not DCA increased p21 expression. Collectively, although both butyrate and DCA inhibit colonic cell proliferation, butyrate increases tumor suppressor gene expression, whereas DCA decreases tumor suppressor activation in cell cycle and apoptosis pathways.

Original languageEnglish (US)
Pages (from-to)1022-1028
Number of pages7
JournalJournal of Nutritional Biochemistry
Volume26
Issue number10
DOIs
StatePublished - Oct 1 2015

Fingerprint

Deoxycholic Acid
Butyrates
Cell proliferation
Colon
Cell Proliferation
Cell signaling
Cell Cycle
Cells
Apoptosis
Metabolites
S Phase
Colonic Neoplasms
Tumors
Chemical activation
Retinoblastoma Protein
Dietary Fiber
High Fat Diet
Nutrition
Tumor Suppressor Genes
Bile Acids and Salts

Keywords

  • Apoptosis
  • Butyrate
  • Cell cycle
  • Colon cancer
  • Deoxycholic acid

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Nutrition and Dietetics
  • Clinical Biochemistry

Cite this

Butyrate and deoxycholic acid play common and distinct roles in HCT116 human colon cell proliferation. / Zeng, Huawei; Claycombe, Kate J.; Reindl, Katie M.

In: Journal of Nutritional Biochemistry, Vol. 26, No. 10, 01.10.2015, p. 1022-1028.

Research output: Contribution to journalArticle

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