Bronchial epithelial cells release monocyte chemotactic activity in response to smoke and endotoxin

S. Koyama, S. I. Rennard, G. D. Leikauf, R. A. Robbins

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

An increase in mononuclear phagocytes occurs within the airways during airway inflammation. Bronchial epithelial cells could release monocyte chemotactic activity and contribute to this increase. To test this hypothesis, bovine bronchial epithelial cells were isolated and maintained in culture. Bronchial epithelial cell culture supernatant fluids were evaluated for monocyte chemotactic activity. Epithelial cell culture supernatant fluids attracted significantly greater numbers of monocytes compared to media alone and the number of monocytes attracted increased in a time dependent manner. Endotoxin and smoke extract induced a dose and time dependent release of monocyte chemotactic activity compared with cells cultured in media (52.5±2.6 (endotoxin), 30.5±2.3 (smoke) vs 20.5±2.2 cells/high power field (HPF) p < 0.001). The released activity was chemotactic by checkerboard analysis. Stimulation of the epithelial cells by opsonized zymosan, calcium ionophore (A23187), and PMA also resulted in an increase in monocyte chemotactic activity (p < 0.01). Because the release of activity was blocked by the lipoxygenase inhibitors, nordihydroguaiaretic acid and diethycarbamazine, epithelial cell monolayers were cultured with 3 μCi [3H]arachidonic acid for 24 h and then exposed to A23187, PMA, or both stimuli, for 4, 8, and 24 h. Analysis of the released 3H activity was performed with reverse-phase HPLC and revealed that the major lipoxygenase product was leukotriene B4. These data suggest that monocytes may be recruited into airways in response to chemotactic factors released by bronchial epithelial cells.

Original languageEnglish (US)
Pages (from-to)972-979
Number of pages8
JournalJournal of Immunology
Volume147
Issue number3
StatePublished - Jan 1 1991

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Endotoxins
Smoke
Monocytes
Epithelial Cells
Calcimycin
Cell Culture Techniques
Masoprocol
Lipoxygenase Inhibitors
Zymosan
Leukotriene B4
Lipoxygenase
Calcium Ionophores
Chemotactic Factors
Phagocytes
Arachidonic Acid
Cultured Cells
High Pressure Liquid Chromatography
Inflammation

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Koyama, S., Rennard, S. I., Leikauf, G. D., & Robbins, R. A. (1991). Bronchial epithelial cells release monocyte chemotactic activity in response to smoke and endotoxin. Journal of Immunology, 147(3), 972-979.

Bronchial epithelial cells release monocyte chemotactic activity in response to smoke and endotoxin. / Koyama, S.; Rennard, S. I.; Leikauf, G. D.; Robbins, R. A.

In: Journal of Immunology, Vol. 147, No. 3, 01.01.1991, p. 972-979.

Research output: Contribution to journalArticle

Koyama, S, Rennard, SI, Leikauf, GD & Robbins, RA 1991, 'Bronchial epithelial cells release monocyte chemotactic activity in response to smoke and endotoxin', Journal of Immunology, vol. 147, no. 3, pp. 972-979.
Koyama, S. ; Rennard, S. I. ; Leikauf, G. D. ; Robbins, R. A. / Bronchial epithelial cells release monocyte chemotactic activity in response to smoke and endotoxin. In: Journal of Immunology. 1991 ; Vol. 147, No. 3. pp. 972-979.
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