Bronchial epithelial cells regulate fibroblast proliferation

Y. Nakamura, L. Tate, R. F. Ertl, M. Kawamoto, T. Mio, Y. Adachi, Debra Romberger, S. Koizumi, G. Gossman, R. A. Robbins, J. R. Spurzem, S. I. Rennard

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

Chronic bronchitis frequently leads to irreversible airway obstruction. Alteration of airway architecture with abnormal airway connective tissue is thought to play an important role in this process. We hypothesized that the epithelial cells that line the airways modulate the development of peribronchial fibrosis and fixed airway obstruction by directing fibroblast proliferation. To assess this, we examined stimulatory activities for human lung fibroblast proliferation in bovine bronchial epithelial cell- conditioned medium. The conditioned medium stimulated the proliferation of fibroblasts in a serum-free culture system in a concentration-dependent manner. The fibroblast growth stimulatory activity was heterogenous, with molecular masses of >50 and ~10 kDa. Bronchial epithelial cell-conditioned medium also contained fibroblast growth inhibitory factors, including both transforming growth factor (TGF)-β and, based on indomethacin sensitivity, cyclooxygenase products. TGF-β appeared to contribute to the morphological change of fibroblasts induced by the conditioned medium. Co-culture of human lung fibroblasts with bronchial epithelial cells resulted in a stimulation of fibroblast proliferation. In summary, airway epithelial cells appear to regulate fibroblast proliferation and may play a role in peribronchial fibrosis in chronic bronchitis.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume269
Issue number3 13-3
StatePublished - Jan 1 1995

Fingerprint

fibroblasts
epithelial cells
Fibroblasts
Epithelial Cells
Conditioned Culture Medium
bronchitis
Chronic Bronchitis
transforming growth factors
Transforming Growth Factors
Airway Obstruction
fibrosis
Fibrosis
lungs
Lung
Fibroblast Growth Factors
Prostaglandin-Endoperoxide Synthases
indomethacin
Coculture Techniques
Human Activities
Indomethacin

Keywords

  • growth factors
  • prostaglandin E
  • transforming growth factor-β

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology

Cite this

Nakamura, Y., Tate, L., Ertl, R. F., Kawamoto, M., Mio, T., Adachi, Y., ... Rennard, S. I. (1995). Bronchial epithelial cells regulate fibroblast proliferation. American Journal of Physiology - Lung Cellular and Molecular Physiology, 269(3 13-3).

Bronchial epithelial cells regulate fibroblast proliferation. / Nakamura, Y.; Tate, L.; Ertl, R. F.; Kawamoto, M.; Mio, T.; Adachi, Y.; Romberger, Debra; Koizumi, S.; Gossman, G.; Robbins, R. A.; Spurzem, J. R.; Rennard, S. I.

In: American Journal of Physiology - Lung Cellular and Molecular Physiology, Vol. 269, No. 3 13-3, 01.01.1995.

Research output: Contribution to journalArticle

Nakamura, Y, Tate, L, Ertl, RF, Kawamoto, M, Mio, T, Adachi, Y, Romberger, D, Koizumi, S, Gossman, G, Robbins, RA, Spurzem, JR & Rennard, SI 1995, 'Bronchial epithelial cells regulate fibroblast proliferation', American Journal of Physiology - Lung Cellular and Molecular Physiology, vol. 269, no. 3 13-3.
Nakamura Y, Tate L, Ertl RF, Kawamoto M, Mio T, Adachi Y et al. Bronchial epithelial cells regulate fibroblast proliferation. American Journal of Physiology - Lung Cellular and Molecular Physiology. 1995 Jan 1;269(3 13-3).
Nakamura, Y. ; Tate, L. ; Ertl, R. F. ; Kawamoto, M. ; Mio, T. ; Adachi, Y. ; Romberger, Debra ; Koizumi, S. ; Gossman, G. ; Robbins, R. A. ; Spurzem, J. R. ; Rennard, S. I. / Bronchial epithelial cells regulate fibroblast proliferation. In: American Journal of Physiology - Lung Cellular and Molecular Physiology. 1995 ; Vol. 269, No. 3 13-3.
@article{5833be59e63b4a968f0f6b79d3054f5d,
title = "Bronchial epithelial cells regulate fibroblast proliferation",
abstract = "Chronic bronchitis frequently leads to irreversible airway obstruction. Alteration of airway architecture with abnormal airway connective tissue is thought to play an important role in this process. We hypothesized that the epithelial cells that line the airways modulate the development of peribronchial fibrosis and fixed airway obstruction by directing fibroblast proliferation. To assess this, we examined stimulatory activities for human lung fibroblast proliferation in bovine bronchial epithelial cell- conditioned medium. The conditioned medium stimulated the proliferation of fibroblasts in a serum-free culture system in a concentration-dependent manner. The fibroblast growth stimulatory activity was heterogenous, with molecular masses of >50 and ~10 kDa. Bronchial epithelial cell-conditioned medium also contained fibroblast growth inhibitory factors, including both transforming growth factor (TGF)-β and, based on indomethacin sensitivity, cyclooxygenase products. TGF-β appeared to contribute to the morphological change of fibroblasts induced by the conditioned medium. Co-culture of human lung fibroblasts with bronchial epithelial cells resulted in a stimulation of fibroblast proliferation. In summary, airway epithelial cells appear to regulate fibroblast proliferation and may play a role in peribronchial fibrosis in chronic bronchitis.",
keywords = "growth factors, prostaglandin E, transforming growth factor-β",
author = "Y. Nakamura and L. Tate and Ertl, {R. F.} and M. Kawamoto and T. Mio and Y. Adachi and Debra Romberger and S. Koizumi and G. Gossman and Robbins, {R. A.} and Spurzem, {J. R.} and Rennard, {S. I.}",
year = "1995",
month = "1",
day = "1",
language = "English (US)",
volume = "269",
journal = "American Journal of Physiology - Renal Physiology",
issn = "0363-6127",
publisher = "American Physiological Society",
number = "3 13-3",

}

TY - JOUR

T1 - Bronchial epithelial cells regulate fibroblast proliferation

AU - Nakamura, Y.

AU - Tate, L.

AU - Ertl, R. F.

AU - Kawamoto, M.

AU - Mio, T.

AU - Adachi, Y.

AU - Romberger, Debra

AU - Koizumi, S.

AU - Gossman, G.

AU - Robbins, R. A.

AU - Spurzem, J. R.

AU - Rennard, S. I.

PY - 1995/1/1

Y1 - 1995/1/1

N2 - Chronic bronchitis frequently leads to irreversible airway obstruction. Alteration of airway architecture with abnormal airway connective tissue is thought to play an important role in this process. We hypothesized that the epithelial cells that line the airways modulate the development of peribronchial fibrosis and fixed airway obstruction by directing fibroblast proliferation. To assess this, we examined stimulatory activities for human lung fibroblast proliferation in bovine bronchial epithelial cell- conditioned medium. The conditioned medium stimulated the proliferation of fibroblasts in a serum-free culture system in a concentration-dependent manner. The fibroblast growth stimulatory activity was heterogenous, with molecular masses of >50 and ~10 kDa. Bronchial epithelial cell-conditioned medium also contained fibroblast growth inhibitory factors, including both transforming growth factor (TGF)-β and, based on indomethacin sensitivity, cyclooxygenase products. TGF-β appeared to contribute to the morphological change of fibroblasts induced by the conditioned medium. Co-culture of human lung fibroblasts with bronchial epithelial cells resulted in a stimulation of fibroblast proliferation. In summary, airway epithelial cells appear to regulate fibroblast proliferation and may play a role in peribronchial fibrosis in chronic bronchitis.

AB - Chronic bronchitis frequently leads to irreversible airway obstruction. Alteration of airway architecture with abnormal airway connective tissue is thought to play an important role in this process. We hypothesized that the epithelial cells that line the airways modulate the development of peribronchial fibrosis and fixed airway obstruction by directing fibroblast proliferation. To assess this, we examined stimulatory activities for human lung fibroblast proliferation in bovine bronchial epithelial cell- conditioned medium. The conditioned medium stimulated the proliferation of fibroblasts in a serum-free culture system in a concentration-dependent manner. The fibroblast growth stimulatory activity was heterogenous, with molecular masses of >50 and ~10 kDa. Bronchial epithelial cell-conditioned medium also contained fibroblast growth inhibitory factors, including both transforming growth factor (TGF)-β and, based on indomethacin sensitivity, cyclooxygenase products. TGF-β appeared to contribute to the morphological change of fibroblasts induced by the conditioned medium. Co-culture of human lung fibroblasts with bronchial epithelial cells resulted in a stimulation of fibroblast proliferation. In summary, airway epithelial cells appear to regulate fibroblast proliferation and may play a role in peribronchial fibrosis in chronic bronchitis.

KW - growth factors

KW - prostaglandin E

KW - transforming growth factor-β

UR - http://www.scopus.com/inward/record.url?scp=0028869625&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028869625&partnerID=8YFLogxK

M3 - Article

VL - 269

JO - American Journal of Physiology - Renal Physiology

JF - American Journal of Physiology - Renal Physiology

SN - 0363-6127

IS - 3 13-3

ER -