Broad-spectrum protection against tombusviruses elicited by defective interfering RNAs in transgenic plants

Teresa Rubio, Marisé Borja, Herman B. Scholthof, Paul A. Feldstein, T. Jack Morris, Andrew O. Jackson

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We have designed a DNA cassette to transcribe defective interfering (DI) RNAs of tomato bushy stunt virus (TBSV) and have investigated their potential to protect transgenic Nicotiana benthamiana plants from tombusvirus infections. To produce RNAs with authentic 5' and 3' termini identical to those of the native B10 DI RNA, the DI RNA sequences were flanked by ribozymes (RzDI). When RzDI RNAs transcribed in vitro were mixed with parental TBSV transcripts and inoculated into protoplasts or plants, they became amplified, reduced the accumulation of the parental RNA, and mediated attenuation of the lethal syndrome characteristic of TBSV infections. Analysis of F1 and F2 RzDI transformants indicated that uninfected plants expressed the DI RNAs in low abundance, but these RNAs were amplified to very high levels during TBSV infection. By two weeks postinoculation with TBSV, all untransformed N. benthamiana plants and transformed negative controls died. Although infection of transgenic RzDI plants initially induced moderate to severe symptoms, these plants subsequently recovered, flowered, and set seed. Plants from the same transgenic lines also exhibited broad-spectrum protection against related tombusviruses but remained susceptible to a distantly related tombus-like virus and to unrelated viruses.

Original languageEnglish (US)
Pages (from-to)5070-5078
Number of pages9
JournalJournal of virology
Volume73
Issue number6
StatePublished - Jun 7 1999

Fingerprint

Tombusvirus
Tomato bushy stunt virus
Genetically Modified Plants
transgenic plants
RNA
Nicotiana benthamiana
Virus Diseases
infection
genetically modified organisms
Viruses
viruses
Catalytic RNA
Protoplasts
Infection
seed set
lethal genes
signs and symptoms (plants)
protoplasts
Tobacco
Seeds

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Rubio, T., Borja, M., Scholthof, H. B., Feldstein, P. A., Morris, T. J., & Jackson, A. O. (1999). Broad-spectrum protection against tombusviruses elicited by defective interfering RNAs in transgenic plants. Journal of virology, 73(6), 5070-5078.

Broad-spectrum protection against tombusviruses elicited by defective interfering RNAs in transgenic plants. / Rubio, Teresa; Borja, Marisé; Scholthof, Herman B.; Feldstein, Paul A.; Morris, T. Jack; Jackson, Andrew O.

In: Journal of virology, Vol. 73, No. 6, 07.06.1999, p. 5070-5078.

Research output: Contribution to journalArticle

Rubio, T, Borja, M, Scholthof, HB, Feldstein, PA, Morris, TJ & Jackson, AO 1999, 'Broad-spectrum protection against tombusviruses elicited by defective interfering RNAs in transgenic plants', Journal of virology, vol. 73, no. 6, pp. 5070-5078.
Rubio T, Borja M, Scholthof HB, Feldstein PA, Morris TJ, Jackson AO. Broad-spectrum protection against tombusviruses elicited by defective interfering RNAs in transgenic plants. Journal of virology. 1999 Jun 7;73(6):5070-5078.
Rubio, Teresa ; Borja, Marisé ; Scholthof, Herman B. ; Feldstein, Paul A. ; Morris, T. Jack ; Jackson, Andrew O. / Broad-spectrum protection against tombusviruses elicited by defective interfering RNAs in transgenic plants. In: Journal of virology. 1999 ; Vol. 73, No. 6. pp. 5070-5078.
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