Brain regional levels of adenosine and adenosine nucleotides in rats killed by high-energy focused microwave irradiation

Suzanne M. Delaney, Jonathan Geiger

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

A high-energy focused microwave system for killing experimental animals was used to rapidly inactivate enzymes and prevent postmortem breakdown of adenine nucleotides and adenosine, thereby enabling accurate measurements of AMP, ADP, ATP and adenosine in rat brain. For comparison, purine levels were measured in brains of rats killed by decapitation, decapitation into liquid nitrogen, or in situ freezing of the brain with liquid nitrogen. Of the three microwave irradiation power levels used, 10, 6.0 or 3.5 kW, rats killed by 10 kW had the highest ATP levels (28.8 nmol/mg protein) and cellular energy charge value (0.8), and the lowest levels of AMP (2.2 nmol/mg protein) and adenosine (19.7 pmol/mg protein). Of the 6 brain regions studied, adenosine levels (pmol/mg protein) ranged from 10 in cerebral cortex to 170 in cerebellum of rats killed using 10 kW microwave irradiation and, for comparison, ranged from 840 in cerebral cortex to 2498 in striatum of rats killed by decapitation. Focused microwave killing permits precise and accurate measurements of purines in discrete regions of rat brain.

Original languageEnglish (US)
Pages (from-to)151-156
Number of pages6
JournalJournal of Neuroscience Methods
Volume64
Issue number2
DOIs
StatePublished - Jan 1 1996
Externally publishedYes

Fingerprint

Microwaves
Adenosine
Nucleotides
Decapitation
Brain
Adenosine Monophosphate
Cerebral Cortex
Proteins
Nitrogen
Adenosine Triphosphate
Purines
Adenine Nucleotides
Adenosine Diphosphate
Cerebellum
Freezing
Enzymes

Keywords

  • Adenine nucleotides
  • Adenosine
  • Brain
  • Decapitation
  • Energy charge
  • Focused microwave killing

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Brain regional levels of adenosine and adenosine nucleotides in rats killed by high-energy focused microwave irradiation. / Delaney, Suzanne M.; Geiger, Jonathan.

In: Journal of Neuroscience Methods, Vol. 64, No. 2, 01.01.1996, p. 151-156.

Research output: Contribution to journalArticle

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