Brain region mapping using global metabolomics

Julijana Ivanisevic, Adrian A. Epstein, Michael E. Kurczy, Paul H. Benton, Winnie Uritboonthai, Howard S Fox, Michael D. Boska, Howard Eliot Gendelman, Gary Siuzdak

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Historically, studies of brain metabolism have been based on targeted analyses of a limited number of metabolites. Here we present an untargeted mass spectrometry-based metabolomic strategy that has successfully uncovered differences in a broad array of metabolites across anatomical regions of the mouse brain. The NSG immunodeficient mouse model was chosen because of its ability to undergo humanization leading to numerous applications in oncology and infectious disease research. Metabolic phenotyping by hydrophilic interaction liquid chromatography and nanostructure imaging mass spectrometry revealed both water-soluble and lipid metabolite patterns across brain regions. Neurochemical differences in metabolic phenotypes were mainly defined by various phospholipids and several intriguing metabolites including carnosine, cholesterol sulfate, lipoamino acids, uric acid, and sialic acid, whose physiological roles in brain metabolism are poorly understood. This study helps define regional homeostasis for the normal mouse brain to give context to the reaction to pathological events.

Original languageEnglish (US)
Pages (from-to)1575-1584
Number of pages10
JournalChemistry and Biology
Volume21
Issue number11
DOIs
StatePublished - Nov 20 2014

Fingerprint

Brain Mapping
Metabolomics
Brain
Metabolites
Metabolism
Mass spectrometry
Mass Spectrometry
Carnosine
Oncology
Nanostructures
Liquid chromatography
N-Acetylneuraminic Acid
Uric Acid
Hydrophobic and Hydrophilic Interactions
Liquid Chromatography
Communicable Diseases
Phospholipids
Homeostasis
Phenotype
Lipids

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

Cite this

Ivanisevic, J., Epstein, A. A., Kurczy, M. E., Benton, P. H., Uritboonthai, W., Fox, H. S., ... Siuzdak, G. (2014). Brain region mapping using global metabolomics. Chemistry and Biology, 21(11), 1575-1584. https://doi.org/10.1016/j.chembiol.2014.09.016

Brain region mapping using global metabolomics. / Ivanisevic, Julijana; Epstein, Adrian A.; Kurczy, Michael E.; Benton, Paul H.; Uritboonthai, Winnie; Fox, Howard S; Boska, Michael D.; Gendelman, Howard Eliot; Siuzdak, Gary.

In: Chemistry and Biology, Vol. 21, No. 11, 20.11.2014, p. 1575-1584.

Research output: Contribution to journalArticle

Ivanisevic, J, Epstein, AA, Kurczy, ME, Benton, PH, Uritboonthai, W, Fox, HS, Boska, MD, Gendelman, HE & Siuzdak, G 2014, 'Brain region mapping using global metabolomics', Chemistry and Biology, vol. 21, no. 11, pp. 1575-1584. https://doi.org/10.1016/j.chembiol.2014.09.016
Ivanisevic J, Epstein AA, Kurczy ME, Benton PH, Uritboonthai W, Fox HS et al. Brain region mapping using global metabolomics. Chemistry and Biology. 2014 Nov 20;21(11):1575-1584. https://doi.org/10.1016/j.chembiol.2014.09.016
Ivanisevic, Julijana ; Epstein, Adrian A. ; Kurczy, Michael E. ; Benton, Paul H. ; Uritboonthai, Winnie ; Fox, Howard S ; Boska, Michael D. ; Gendelman, Howard Eliot ; Siuzdak, Gary. / Brain region mapping using global metabolomics. In: Chemistry and Biology. 2014 ; Vol. 21, No. 11. pp. 1575-1584.
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