Bovine viral diarrhea virus-specific neutralizing antibodies induced by anti-idiotypic antibodies

D. V. Onisk, R. Donis, C. L. Kelling, S. Srikumaran

Research output: Contribution to journalArticle

2 Scopus citations


Two murine neutralizing monoclonal antibodies (MAbs), 4D8 and 6D11, recognizing epitopes on gp53, a surface glycoprotein of bovine viral diarrhea virus (BVDV), were used to generate anti-idiotypic antibodies (anti-ids) in a calf. The polyclonal anti-ids were isolated from serum by affinity chromatography on their respective Ab-1-Sepharose columns, followed by repeated adsorption on isotype-matched antibody-Sepharose columns. The anti-ids reacted specifically with their respective Ab-1, but not with isotype-matched controls. They also inhibited the binding of their Ab-1 to BVDV in a concentration-dependent manner. Mice immunized with the two anti-id preparations developed antibodies to BVDV, which neutralized the virus in vitro.

Original languageEnglish (US)
Pages (from-to)257-263
Number of pages7
JournalViral Immunology
Issue number4
StatePublished - Jan 1 1992


ASJC Scopus subject areas

  • Immunology
  • Molecular Medicine
  • Virology

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