Bovine respiratory syncytial virus in-situ hybridization from sheep lungs at different times postinfection

E. Redondo, L. Gomez, Clayton L Kelling, A. Gazquez, A. J. Masot

Research output: Contribution to journalArticle

Abstract

We studied the distribution of bovine respiratory syncytial virus (BRSV) RNA in lungs of experimentally infected sheep by in situ-hybridization at different times postinfection. The probe used for in-situ hybridization was prepared by reverse transcription of BRSV RNA, followed by PCR amplification of the cDNA. Twenty five Merino sheeps of both sexes with a live weight of 55± 10 Kg, received a intratracheal inoculation of 40 ml saline solution containing 1.26 × 106 TCID50 BRSV (strain NMK7) per ml. Sheep were slaughtered 1,3,7,11 and 15 postinoculation days (PID). Bronchial and bronchiolar epithelial cells were positive for BRSV nucleic acid by ISH at 1, 3, 7 and 11 PID. However, alveolar epithelial cells contained positive hybridization signals cells at 1,3 and 7 PID. Cells containing viral RNA were detected from 1 to 11 PID, in exudate within bronchial and bronchiolar lumina; and from 3 to 7 PID in alveolar exudates. Positive hybridization signals were identified in interstitial mononuclear cells and in bronchi associated lymphoid tissue from 3 to 11 PID. The highest signal intensity of positive cells were observed at 3 and 7 PID, coinciding with high virus antibodies titres and with the most important histopathological findings.

Original languageEnglish (US)
Pages (from-to)37-49
Number of pages13
JournalArchivos de Medicina Veterinaria
Volume35
Issue number1
DOIs
StatePublished - Jan 1 2003

Fingerprint

Bovine respiratory syncytial virus
in situ hybridization
In Situ Hybridization
Sheep
lungs
sheep
Lung
RNA
Exudates and Transudates
epithelial cells
hybridization
cells
Alveolar Epithelial Cells
bronchi
reverse transcription
Viral RNA
Lymphoid Tissue
Bronchi
Merino
Viral Load

Keywords

  • Bovine respiratory syncytial virus
  • In situ hybridization
  • Lungs
  • Sheep

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Bovine respiratory syncytial virus in-situ hybridization from sheep lungs at different times postinfection. / Redondo, E.; Gomez, L.; Kelling, Clayton L; Gazquez, A.; Masot, A. J.

In: Archivos de Medicina Veterinaria, Vol. 35, No. 1, 01.01.2003, p. 37-49.

Research output: Contribution to journalArticle

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