Bovine lactoferrin mRNA: Sequence, analysis, and expression in the mammary gland

Richard E. Goodman, Floyd L. Schanbacher

Research output: Contribution to journalArticle

86 Citations (Scopus)

Abstract

The mRNA sequence for bovine lactoferrin expressed in the mammary gland was determined by sequencing three over lapping cDNA clones and by direct sequencing of the mRNA. The mRNA (2351 bases) codes for a 708 amino acid protein with a 19 amino acid signal peptide immediately preceding a sequence identical to the N-terminal 40 amino acids reported for bovine lactoferrin (1). A putative destabilizing sequence (AUUUA) was identified in the 3′-untranslated region. The nucleic acid sequence and deduced amino acid sequence are highly homologous with other transferrin family members. Lactoferrin mRNA concentrations in bovine mammary tissue were quite low two days before parturition and during lactation but were high three days after the cessation of milking, a sharp contrast from the pattern of regulation of the other milk proteins.

Original languageEnglish (US)
Pages (from-to)75-84
Number of pages10
JournalBiochemical and Biophysical Research Communications
Volume180
Issue number1
DOIs
StatePublished - Oct 15 1991

Fingerprint

Lactoferrin
Human Mammary Glands
Sequence Analysis
Amino Acids
Messenger RNA
Nucleic acid sequences
Lapping
Amino Acid Sequence Homology
Milk Proteins
3' Untranslated Regions
Transferrin
Protein Sorting Signals
Lactation
Nucleic Acids
Breast
Complementary DNA
Clone Cells
Parturition
Tissue
Proteins

Keywords

  • aa
  • amino acid
  • bLf
  • bovine lactoferrin
  • hLf
  • hTf
  • human lactoferrin
  • human transferrin
  • mLf
  • mouse lactoferrin
  • oTf,
  • ovotransferrin
  • pTf
  • porcine transferrin

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Bovine lactoferrin mRNA : Sequence, analysis, and expression in the mammary gland. / Goodman, Richard E.; Schanbacher, Floyd L.

In: Biochemical and Biophysical Research Communications, Vol. 180, No. 1, 15.10.1991, p. 75-84.

Research output: Contribution to journalArticle

@article{160662a96933469aab16b439894a115f,
title = "Bovine lactoferrin mRNA: Sequence, analysis, and expression in the mammary gland",
abstract = "The mRNA sequence for bovine lactoferrin expressed in the mammary gland was determined by sequencing three over lapping cDNA clones and by direct sequencing of the mRNA. The mRNA (2351 bases) codes for a 708 amino acid protein with a 19 amino acid signal peptide immediately preceding a sequence identical to the N-terminal 40 amino acids reported for bovine lactoferrin (1). A putative destabilizing sequence (AUUUA) was identified in the 3′-untranslated region. The nucleic acid sequence and deduced amino acid sequence are highly homologous with other transferrin family members. Lactoferrin mRNA concentrations in bovine mammary tissue were quite low two days before parturition and during lactation but were high three days after the cessation of milking, a sharp contrast from the pattern of regulation of the other milk proteins.",
keywords = "aa, amino acid, bLf, bovine lactoferrin, hLf, hTf, human lactoferrin, human transferrin, mLf, mouse lactoferrin, oTf,, ovotransferrin, pTf, porcine transferrin",
author = "Goodman, {Richard E.} and Schanbacher, {Floyd L.}",
year = "1991",
month = "10",
day = "15",
doi = "10.1016/S0006-291X(05)81257-4",
language = "English (US)",
volume = "180",
pages = "75--84",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Bovine lactoferrin mRNA

T2 - Sequence, analysis, and expression in the mammary gland

AU - Goodman, Richard E.

AU - Schanbacher, Floyd L.

PY - 1991/10/15

Y1 - 1991/10/15

N2 - The mRNA sequence for bovine lactoferrin expressed in the mammary gland was determined by sequencing three over lapping cDNA clones and by direct sequencing of the mRNA. The mRNA (2351 bases) codes for a 708 amino acid protein with a 19 amino acid signal peptide immediately preceding a sequence identical to the N-terminal 40 amino acids reported for bovine lactoferrin (1). A putative destabilizing sequence (AUUUA) was identified in the 3′-untranslated region. The nucleic acid sequence and deduced amino acid sequence are highly homologous with other transferrin family members. Lactoferrin mRNA concentrations in bovine mammary tissue were quite low two days before parturition and during lactation but were high three days after the cessation of milking, a sharp contrast from the pattern of regulation of the other milk proteins.

AB - The mRNA sequence for bovine lactoferrin expressed in the mammary gland was determined by sequencing three over lapping cDNA clones and by direct sequencing of the mRNA. The mRNA (2351 bases) codes for a 708 amino acid protein with a 19 amino acid signal peptide immediately preceding a sequence identical to the N-terminal 40 amino acids reported for bovine lactoferrin (1). A putative destabilizing sequence (AUUUA) was identified in the 3′-untranslated region. The nucleic acid sequence and deduced amino acid sequence are highly homologous with other transferrin family members. Lactoferrin mRNA concentrations in bovine mammary tissue were quite low two days before parturition and during lactation but were high three days after the cessation of milking, a sharp contrast from the pattern of regulation of the other milk proteins.

KW - aa

KW - amino acid

KW - bLf

KW - bovine lactoferrin

KW - hLf

KW - hTf

KW - human lactoferrin

KW - human transferrin

KW - mLf

KW - mouse lactoferrin

KW - oTf,

KW - ovotransferrin

KW - pTf

KW - porcine transferrin

UR - http://www.scopus.com/inward/record.url?scp=0026086647&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026086647&partnerID=8YFLogxK

U2 - 10.1016/S0006-291X(05)81257-4

DO - 10.1016/S0006-291X(05)81257-4

M3 - Article

C2 - 1718281

AN - SCOPUS:0026086647

VL - 180

SP - 75

EP - 84

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -