Bovine kappa-casein gene polymorphism and its association with milk production traits

Satyanarayana Rachagani, Ishwar Dayal Gupta

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Point mutations in exon IV of the bovine κ-casein (CSN3) gene determine two allelic variants, A and B. These variants were distinguished by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis in the indigenous Sahiwal and Tharparkar cattle breeds. DNA samples (252 Sahiwal and 56 Tharparkar) were analyzed for allelic variants of the CSN3 gene. Polymorphism was detected by digestion of PCR-amplified products with HindIII, HhaI and HaeIII restriction enzymes, followed by separation on 3% agarose gels, and resolved by ethidium bromide staining. Allele A of the κ-casein gene occurred at a higher frequency than allele B, in both Sahiwal and Tharparkar breeds. The genotypic frequencies of AA, AB, and BB in the Sahiwal and Tharparkar breeds were 0.758, 0.230 and 0.012, and 0.0.732, 0.250 and 0.018, respectively. The frequencies of alleles A and B in the Sahiwal and Tharparkar breeds were 0.873 and 0.127, and 0.857 and 0.143, respectively. Genotype BB of the kappa-casein gene had more influence on the monthly milk yield, 305-days milk yield, monthly solids-not-fat (SNF) yield, and monthly protein yield, in the Sahiwal cattle.

Original languageEnglish (US)
Pages (from-to)893-897
Number of pages5
JournalGenetics and Molecular Biology
Volume31
Issue number4
DOIs
StatePublished - Jan 1 2008

Fingerprint

Caseins
Milk
Gene Frequency
Genes
Polymerase Chain Reaction
Ethidium
Point Mutation
Restriction Fragment Length Polymorphisms
Sepharose
Digestion
Exons
Gels
Fats
Alleles
Genotype
Staining and Labeling
DNA
Enzymes
Proteins

Keywords

  • Genetic polymorphism
  • Indian cattle
  • K-casein
  • R-RFLP

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Bovine kappa-casein gene polymorphism and its association with milk production traits. / Rachagani, Satyanarayana; Gupta, Ishwar Dayal.

In: Genetics and Molecular Biology, Vol. 31, No. 4, 01.01.2008, p. 893-897.

Research output: Contribution to journalArticle

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