Bovine herpesvirus 1 productive infection stimulates inflammasome formation and caspase 1 activity

Jianlin Wang, Jeff Alexander, Matthew Wiebe, Clinton Jones

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Bovine herpesvirus 1 (BoHV-1), a significant viral pathogen of cattle, causes inflammation in affected tissue during acute infection. Consequently, we tested whether productively infected bovine cells stimulate inflammasome formation. Expression of two components required for inflammasome formation, the DNA sensor IFI16 (gamma-interferon-inducible protein 16) and NLRP3 (NOD-like receptor family, pyrin domain containing 3), were induced in bovine kidney cells by eight hours after infection. IFI16 was detected in punctate granules localized to the cytoplasm and nucleus. During productive infection, more than ten times more cells were caspase 1 positive, which is activated following inflammasome formation. Two caspase 1 inhibitors had no effect on productive infection. Conversely, another caspase 1 inhibitor, glyburide, significantly inhibited virus infection suggesting it had off-target effects on related enzymes or interfered with infection via non-enzymatic mechanisms. Collectively, these studies demonstrated that BoHV-1 infection stimulated inflammasome formation, which we predict is important for clinical symptoms in cattle.

Original languageEnglish (US)
Pages (from-to)72-76
Number of pages5
JournalVirus Research
Volume185
DOIs
StatePublished - Jun 24 2014

Fingerprint

Bovine Herpesvirus 1
Inflammasomes
Caspase 1
Infection
Interferon-gamma
Herpesviridae Infections
Glyburide
Virus Diseases
Cytoplasm
Inflammation
Kidney
DNA
Enzymes
Proteins

Keywords

  • BoHV-1
  • Caspase 1
  • DNA sensor IFI16
  • Inflammasome
  • NLRP3

ASJC Scopus subject areas

  • Cancer Research
  • Virology
  • Infectious Diseases

Cite this

Bovine herpesvirus 1 productive infection stimulates inflammasome formation and caspase 1 activity. / Wang, Jianlin; Alexander, Jeff; Wiebe, Matthew; Jones, Clinton.

In: Virus Research, Vol. 185, 24.06.2014, p. 72-76.

Research output: Contribution to journalArticle

Wang, Jianlin ; Alexander, Jeff ; Wiebe, Matthew ; Jones, Clinton. / Bovine herpesvirus 1 productive infection stimulates inflammasome formation and caspase 1 activity. In: Virus Research. 2014 ; Vol. 185. pp. 72-76.
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