Bovine herpesvirus 1 productive infection and immediate early transcription unit 1 promoter are stimulated by the synthetic corticosteroid dexamethasone

Insun Kook, Caitlin Henley, Florencia Meyer, Federico G. Hoffmann, Clinton Jones

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The primary site for life-long latency of bovine herpesvirus 1 (BHV-1) is sensory neurons. The synthetic corticosteroid dexamethasone consistently induces reactivation from latency; however the mechanism by which corticosteroids mediate reactivation is unclear. In this study, we demonstrate for the first time that dexamethasone stimulates productive infection, in part, because the BHV-1 genome contains more than 100 potential glucocorticoid receptor (GR) response elements (GREs). Immediate early transcription unit 1 (IEtu1) promoter activity, but not IEtu2 or VP16 promoter activity, was stimulated by dexamethasone. Two near perfect consensus GREs located within the IEtu1 promoter were necessary for dexamethasone-mediated stimulation. Electrophoretic mobility shift assays and chromatin immunoprecipitation studies demonstrated that the GR interacts with IEtu1 promoter sequences containing the GREs. Although we hypothesize that DEX-mediated stimulation of IEtu1 promoter activity is important during productive infection and perhaps reactivation from latency, stress likely has pleiotropic effects on virus-infected cells.

Original languageEnglish (US)
Pages (from-to)377-385
Number of pages9
JournalVirology
Volume484
DOIs
StatePublished - Oct 1 2015

Fingerprint

Bovine Herpesvirus 1
Dexamethasone
Adrenal Cortex Hormones
Response Elements
Glucocorticoid Receptors
Infection
Chromatin Immunoprecipitation
Electrophoretic Mobility Shift Assay
Sensory Receptor Cells
Genome
Viruses

Keywords

  • BHV-1
  • Glucocorticoid receptor
  • Glucocorticoid response element
  • Immediate early transcription unit 1
  • Stress-induced viral replication

ASJC Scopus subject areas

  • Virology

Cite this

Bovine herpesvirus 1 productive infection and immediate early transcription unit 1 promoter are stimulated by the synthetic corticosteroid dexamethasone. / Kook, Insun; Henley, Caitlin; Meyer, Florencia; Hoffmann, Federico G.; Jones, Clinton.

In: Virology, Vol. 484, 01.10.2015, p. 377-385.

Research output: Contribution to journalArticle

Kook, Insun ; Henley, Caitlin ; Meyer, Florencia ; Hoffmann, Federico G. ; Jones, Clinton. / Bovine herpesvirus 1 productive infection and immediate early transcription unit 1 promoter are stimulated by the synthetic corticosteroid dexamethasone. In: Virology. 2015 ; Vol. 484. pp. 377-385.
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