Bovine bronchial epithelial cells metabolize L-arginine to L-citrulline: Possible role of nitric oxide synthase

R. A. Robbins, F. G. Hamel, A. A. Floreani, G. L. Gossman, K. J. Nelson, S. Belenky, I. Rubinstein

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Abstract

The conversion of L-arginine to L-citrulline is catalyzed by nitric oxide synthase (NOS), and results in the release of nitric oxide (NO). We hypothesized that bronchial epithelial cells metabolize L-arginine to L-citrulline. We found that cell lysates obtained from unstimulated, cultured bovine bronchial epithelial cells (BBECs) converted L-[3H]arginine to L-[3H]citrulline. This conversion was attenuated by three competitive NOS inhibitors and modulated by lipopolysaccharide and cigarette smoke extract (p<0.01, all comparisons). These data demonstrate that BBECs metabolize L-arginine to L-citrulline and implicate a role for the L-arginine:NOS biosynthetic pathway in modulating airway responses.

Original languageEnglish (US)
Pages (from-to)709-716
Number of pages8
JournalLife Sciences
Volume52
Issue number8
DOIs
StatePublished - 1993

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Robbins, R. A., Hamel, F. G., Floreani, A. A., Gossman, G. L., Nelson, K. J., Belenky, S., & Rubinstein, I. (1993). Bovine bronchial epithelial cells metabolize L-arginine to L-citrulline: Possible role of nitric oxide synthase. Life Sciences, 52(8), 709-716. https://doi.org/10.1016/0024-3205(93)90232-R