Bovine bronchial epithelial cells metabolize L-arginine to L-citrulline

Possible role of nitric oxide synthase

R. A. Robbins, Frederick G Hamel, A. A. Floreani, G. L. Gossman, K. J. Nelson, S. Belenky, I. Rubinstein

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

The conversion of L-arginine to L-citrulline is catalyzed by nitric oxide synthase (NOS), and results in the release of nitric oxide (NO). We hypothesized that bronchial epithelial cells metabolize L-arginine to L-citrulline. We found that cell lysates obtained from unstimulated, cultured bovine bronchial epithelial cells (BBECs) converted L-[3H]arginine to L-[3H]citrulline. This conversion was attenuated by three competitive NOS inhibitors and modulated by lipopolysaccharide and cigarette smoke extract (p<0.01, all comparisons). These data demonstrate that BBECs metabolize L-arginine to L-citrulline and implicate a role for the L-arginine:NOS biosynthetic pathway in modulating airway responses.

Original languageEnglish (US)
Pages (from-to)709-716
Number of pages8
JournalLife Sciences
Volume52
Issue number8
DOIs
StatePublished - Jan 1 1993

Fingerprint

Citrulline
Nitric Oxide Synthase
Arginine
Epithelial Cells
Biosynthetic Pathways
Smoke
Tobacco Products
Lipopolysaccharides
Nitric Oxide

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

Robbins, R. A., Hamel, F. G., Floreani, A. A., Gossman, G. L., Nelson, K. J., Belenky, S., & Rubinstein, I. (1993). Bovine bronchial epithelial cells metabolize L-arginine to L-citrulline: Possible role of nitric oxide synthase. Life Sciences, 52(8), 709-716. https://doi.org/10.1016/0024-3205(93)90232-R

Bovine bronchial epithelial cells metabolize L-arginine to L-citrulline : Possible role of nitric oxide synthase. / Robbins, R. A.; Hamel, Frederick G; Floreani, A. A.; Gossman, G. L.; Nelson, K. J.; Belenky, S.; Rubinstein, I.

In: Life Sciences, Vol. 52, No. 8, 01.01.1993, p. 709-716.

Research output: Contribution to journalArticle

Robbins, RA, Hamel, FG, Floreani, AA, Gossman, GL, Nelson, KJ, Belenky, S & Rubinstein, I 1993, 'Bovine bronchial epithelial cells metabolize L-arginine to L-citrulline: Possible role of nitric oxide synthase', Life Sciences, vol. 52, no. 8, pp. 709-716. https://doi.org/10.1016/0024-3205(93)90232-R
Robbins, R. A. ; Hamel, Frederick G ; Floreani, A. A. ; Gossman, G. L. ; Nelson, K. J. ; Belenky, S. ; Rubinstein, I. / Bovine bronchial epithelial cells metabolize L-arginine to L-citrulline : Possible role of nitric oxide synthase. In: Life Sciences. 1993 ; Vol. 52, No. 8. pp. 709-716.
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