Boron stress activates the general amino acid control mechanism and inhibits protein synthesis

Irem Uluisik, Alaattin Kaya, Dmitri Fomenko, Huseyin C. Karakaya, Bradley A. Carlson, Vadim N. Gladyshev, Ahmet Koc

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Boron is an essential micronutrient for plants, and it is beneficial for animals. However, at high concentrations boron is toxic to cells although the mechanism of this toxicity is not known. Atr1 has recently been identified as a boron efflux pump whose expression is upregulated in response to boron treatment. Here, we found that the expression of ATR1 is associated with expression of genes involved in amino acid biosynthesis. These mechanisms are strictly controlled by the transcription factor Gcn4 in response to boron treatment. Further analyses have shown that boron impaired protein synthesis by promoting phosphorylation of eIF2α in a Gcn2 kinase dependent manner. The uncharged tRNA binding domain (HisRS) of Gcn2 is necessary for the phosphorylation of eIF2α in the presence of boron. We postulate that boron exerts its toxic effect through activation of the general amino acid control system and inhibition of protein synthesis. Since the general amino acid control pathway is conserved among eukaryotes, this mechanism of boron toxicity may be of general importance.

Original languageEnglish (US)
Article numbere27772
JournalPloS one
Volume6
Issue number11
DOIs
StatePublished - Nov 17 2011

Fingerprint

Boron
boron
protein synthesis
Amino Acids
amino acids
Proteins
Phosphorylation
Poisons
Toxicity
phosphorylation
toxicity
Aminoacylation
Micronutrients
Biosynthesis
plant micronutrients
Transfer RNA
Eukaryota
transporters
eukaryotic cells
phosphotransferases (kinases)

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Uluisik, I., Kaya, A., Fomenko, D., Karakaya, H. C., Carlson, B. A., Gladyshev, V. N., & Koc, A. (2011). Boron stress activates the general amino acid control mechanism and inhibits protein synthesis. PloS one, 6(11), [e27772]. https://doi.org/10.1371/journal.pone.0027772

Boron stress activates the general amino acid control mechanism and inhibits protein synthesis. / Uluisik, Irem; Kaya, Alaattin; Fomenko, Dmitri; Karakaya, Huseyin C.; Carlson, Bradley A.; Gladyshev, Vadim N.; Koc, Ahmet.

In: PloS one, Vol. 6, No. 11, e27772, 17.11.2011.

Research output: Contribution to journalArticle

Uluisik, I, Kaya, A, Fomenko, D, Karakaya, HC, Carlson, BA, Gladyshev, VN & Koc, A 2011, 'Boron stress activates the general amino acid control mechanism and inhibits protein synthesis', PloS one, vol. 6, no. 11, e27772. https://doi.org/10.1371/journal.pone.0027772
Uluisik, Irem ; Kaya, Alaattin ; Fomenko, Dmitri ; Karakaya, Huseyin C. ; Carlson, Bradley A. ; Gladyshev, Vadim N. ; Koc, Ahmet. / Boron stress activates the general amino acid control mechanism and inhibits protein synthesis. In: PloS one. 2011 ; Vol. 6, No. 11.
@article{2bec0668a3314abbbc835a46f452b4b9,
title = "Boron stress activates the general amino acid control mechanism and inhibits protein synthesis",
abstract = "Boron is an essential micronutrient for plants, and it is beneficial for animals. However, at high concentrations boron is toxic to cells although the mechanism of this toxicity is not known. Atr1 has recently been identified as a boron efflux pump whose expression is upregulated in response to boron treatment. Here, we found that the expression of ATR1 is associated with expression of genes involved in amino acid biosynthesis. These mechanisms are strictly controlled by the transcription factor Gcn4 in response to boron treatment. Further analyses have shown that boron impaired protein synthesis by promoting phosphorylation of eIF2α in a Gcn2 kinase dependent manner. The uncharged tRNA binding domain (HisRS) of Gcn2 is necessary for the phosphorylation of eIF2α in the presence of boron. We postulate that boron exerts its toxic effect through activation of the general amino acid control system and inhibition of protein synthesis. Since the general amino acid control pathway is conserved among eukaryotes, this mechanism of boron toxicity may be of general importance.",
author = "Irem Uluisik and Alaattin Kaya and Dmitri Fomenko and Karakaya, {Huseyin C.} and Carlson, {Bradley A.} and Gladyshev, {Vadim N.} and Ahmet Koc",
year = "2011",
month = "11",
day = "17",
doi = "10.1371/journal.pone.0027772",
language = "English (US)",
volume = "6",
journal = "PLoS One",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "11",

}

TY - JOUR

T1 - Boron stress activates the general amino acid control mechanism and inhibits protein synthesis

AU - Uluisik, Irem

AU - Kaya, Alaattin

AU - Fomenko, Dmitri

AU - Karakaya, Huseyin C.

AU - Carlson, Bradley A.

AU - Gladyshev, Vadim N.

AU - Koc, Ahmet

PY - 2011/11/17

Y1 - 2011/11/17

N2 - Boron is an essential micronutrient for plants, and it is beneficial for animals. However, at high concentrations boron is toxic to cells although the mechanism of this toxicity is not known. Atr1 has recently been identified as a boron efflux pump whose expression is upregulated in response to boron treatment. Here, we found that the expression of ATR1 is associated with expression of genes involved in amino acid biosynthesis. These mechanisms are strictly controlled by the transcription factor Gcn4 in response to boron treatment. Further analyses have shown that boron impaired protein synthesis by promoting phosphorylation of eIF2α in a Gcn2 kinase dependent manner. The uncharged tRNA binding domain (HisRS) of Gcn2 is necessary for the phosphorylation of eIF2α in the presence of boron. We postulate that boron exerts its toxic effect through activation of the general amino acid control system and inhibition of protein synthesis. Since the general amino acid control pathway is conserved among eukaryotes, this mechanism of boron toxicity may be of general importance.

AB - Boron is an essential micronutrient for plants, and it is beneficial for animals. However, at high concentrations boron is toxic to cells although the mechanism of this toxicity is not known. Atr1 has recently been identified as a boron efflux pump whose expression is upregulated in response to boron treatment. Here, we found that the expression of ATR1 is associated with expression of genes involved in amino acid biosynthesis. These mechanisms are strictly controlled by the transcription factor Gcn4 in response to boron treatment. Further analyses have shown that boron impaired protein synthesis by promoting phosphorylation of eIF2α in a Gcn2 kinase dependent manner. The uncharged tRNA binding domain (HisRS) of Gcn2 is necessary for the phosphorylation of eIF2α in the presence of boron. We postulate that boron exerts its toxic effect through activation of the general amino acid control system and inhibition of protein synthesis. Since the general amino acid control pathway is conserved among eukaryotes, this mechanism of boron toxicity may be of general importance.

UR - http://www.scopus.com/inward/record.url?scp=81255195855&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=81255195855&partnerID=8YFLogxK

U2 - 10.1371/journal.pone.0027772

DO - 10.1371/journal.pone.0027772

M3 - Article

VL - 6

JO - PLoS One

JF - PLoS One

SN - 1932-6203

IS - 11

M1 - e27772

ER -