Booster immunization of HIV-1 negative volunteers with HGP-30 vaccine induces protection against HIV-1 virus challenge in SCID mice

P. S. Sarin, James E Talmadge, P. Heseltine, N. Murcar, Howard Eliot Gendelman, R. Coleman, L. Kelsey, S. Beckner, D. Winship, J. Kahn

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Eleven HIV-1 seronegative subjects previously injected with an HIV-1 p17 synthetic peptide vaccine (HGP-30) were given two booster immunizations to evaluate memory cell responses and the ability to boost cellular and humoral immune responses. Five of 11 subjects showed a significant increase in their antibody titres to HGP-30 or p17 and 6/11 had T-cell proliferation responses to either HGP-30 or p17. HIV-1 virus challenge studies in SCID mice demonstrated that 39 of 50 mice (78%) receiving PBMC from 5 of the HGP-30 immunized subjects were protected from infection with a different strain of HIV-1 compared to 4 of 30 mice (13%) that received PBMC from 3 non-immunized subjects (p < 0.001). These studies show that booster immunizations with HGP- 30 vaccine are safe and non-toxic and induce protective cell mediated immune responses.

Original languageEnglish (US)
Pages (from-to)64-71
Number of pages8
JournalVaccine
Volume17
Issue number1
DOIs
StatePublished - Jan 1 1999

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Secondary Immunization
SCID Mice
Human immunodeficiency virus 1
volunteers
HIV-1
Volunteers
Vaccines
vaccines
Viruses
viruses
mice
mononuclear leukocytes
cell-mediated immunity
synthetic vaccines
Synthetic Vaccines
Subunit Vaccines
subunit vaccines
synthetic peptides
Humoral Immunity
humoral immunity

Keywords

  • HGP-30
  • HIV-1 vaccine
  • SCID mice

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

Cite this

Booster immunization of HIV-1 negative volunteers with HGP-30 vaccine induces protection against HIV-1 virus challenge in SCID mice. / Sarin, P. S.; Talmadge, James E; Heseltine, P.; Murcar, N.; Gendelman, Howard Eliot; Coleman, R.; Kelsey, L.; Beckner, S.; Winship, D.; Kahn, J.

In: Vaccine, Vol. 17, No. 1, 01.01.1999, p. 64-71.

Research output: Contribution to journalArticle

Sarin, P. S. ; Talmadge, James E ; Heseltine, P. ; Murcar, N. ; Gendelman, Howard Eliot ; Coleman, R. ; Kelsey, L. ; Beckner, S. ; Winship, D. ; Kahn, J. / Booster immunization of HIV-1 negative volunteers with HGP-30 vaccine induces protection against HIV-1 virus challenge in SCID mice. In: Vaccine. 1999 ; Vol. 17, No. 1. pp. 64-71.
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