Bone-inducing activity of mature BMP-2b produced from a Hybrid BMP-2a/2b precursor

R. Glenn Hammonds, Ralph Schwall, Andrew T Dudley, Lucy Berkemeier, Cora Lai, James Lee, Noreen Cunningham, A. H. Reddi, William I. Wood, Anthony J. Mason

Research output: Contribution to journalArticle

141 Citations (Scopus)

Abstract

The human osteoinductive proteins BMP-2a and BMP-2b have been cloned and expressed in mammalian cells. In order to improve expression levels we examined the role of the proregion in assembly and export. Use of the BMP-2a proregion combined with the mature region of BMP-2b leads to dramatically improved expression of mature BMP-2b. Mature BMP-2b has been purified to near homogeneity from the BMP-2a/2b hybrid, and its structural properties and biological activity determined. Recombinant mature BMP-2b homodimer elicits bone formation in vivo.

Original languageEnglish (US)
Pages (from-to)149-155
Number of pages7
JournalMolecular Endocrinology
Volume5
Issue number1
DOIs
StatePublished - Jan 1 1991

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Osteogenesis
Bone and Bones
Proteins

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology

Cite this

Hammonds, R. G., Schwall, R., Dudley, A. T., Berkemeier, L., Lai, C., Lee, J., ... Mason, A. J. (1991). Bone-inducing activity of mature BMP-2b produced from a Hybrid BMP-2a/2b precursor. Molecular Endocrinology, 5(1), 149-155. https://doi.org/10.1210/mend-5-1-149

Bone-inducing activity of mature BMP-2b produced from a Hybrid BMP-2a/2b precursor. / Hammonds, R. Glenn; Schwall, Ralph; Dudley, Andrew T; Berkemeier, Lucy; Lai, Cora; Lee, James; Cunningham, Noreen; Reddi, A. H.; Wood, William I.; Mason, Anthony J.

In: Molecular Endocrinology, Vol. 5, No. 1, 01.01.1991, p. 149-155.

Research output: Contribution to journalArticle

Hammonds, RG, Schwall, R, Dudley, AT, Berkemeier, L, Lai, C, Lee, J, Cunningham, N, Reddi, AH, Wood, WI & Mason, AJ 1991, 'Bone-inducing activity of mature BMP-2b produced from a Hybrid BMP-2a/2b precursor', Molecular Endocrinology, vol. 5, no. 1, pp. 149-155. https://doi.org/10.1210/mend-5-1-149
Hammonds, R. Glenn ; Schwall, Ralph ; Dudley, Andrew T ; Berkemeier, Lucy ; Lai, Cora ; Lee, James ; Cunningham, Noreen ; Reddi, A. H. ; Wood, William I. ; Mason, Anthony J. / Bone-inducing activity of mature BMP-2b produced from a Hybrid BMP-2a/2b precursor. In: Molecular Endocrinology. 1991 ; Vol. 5, No. 1. pp. 149-155.
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