BMP4-dependent expression of Xenopus Grainyhead-like 1 is essential for epidermal differentiation

Jianning Tao, Emin Kuliyev, Xi Wang, Xiuling Li, Tomasz Wilanowski, Stephen M. Jane, Paul E. Mead, John M. Cunningham

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Morphogen-dependent epidermal-specific transacting factors have not been defined in vertebrates. We demonstrate that a member of the grainyhead transcription factor family, Grainyhead-like 1 (XGrhl1) is essential for ectodermal ontogeny in Xenopus laevis. Expression of this factor is restricted to epidermal cells. Moreover, XGrhl1 is regulated by the BMP4 signaling cascade. Disruption of XGrhl1 activity in vivo results in a severe defect in terminal epidermal differentiation, with inhibition of XK81A1 epidermal keratin gene expression, a key target of BMP4 signaling. Furthermore, transcription of the XK81A1 gene is modulated directly by binding of XGRHL1 to a promoter-localized binding motif that is essential for high-level expression. These results establish a novel developmental role for XGrhl1 as a crucial tissue-specific regulator of vertebrate epidermal differentiation.

Original languageEnglish (US)
Pages (from-to)1021-1034
Number of pages14
JournalDevelopment
Volume132
Issue number5
DOIs
StatePublished - Mar 1 2005

Fingerprint

Xenopus
Vertebrates
Xenopus laevis
Keratins
Transcription Factors
Gene Expression
Genes

Keywords

  • BMP
  • Grainyhead-like 1
  • Xenopus

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology

Cite this

Tao, J., Kuliyev, E., Wang, X., Li, X., Wilanowski, T., Jane, S. M., ... Cunningham, J. M. (2005). BMP4-dependent expression of Xenopus Grainyhead-like 1 is essential for epidermal differentiation. Development, 132(5), 1021-1034. https://doi.org/10.1242/dev.01641

BMP4-dependent expression of Xenopus Grainyhead-like 1 is essential for epidermal differentiation. / Tao, Jianning; Kuliyev, Emin; Wang, Xi; Li, Xiuling; Wilanowski, Tomasz; Jane, Stephen M.; Mead, Paul E.; Cunningham, John M.

In: Development, Vol. 132, No. 5, 01.03.2005, p. 1021-1034.

Research output: Contribution to journalArticle

Tao, J, Kuliyev, E, Wang, X, Li, X, Wilanowski, T, Jane, SM, Mead, PE & Cunningham, JM 2005, 'BMP4-dependent expression of Xenopus Grainyhead-like 1 is essential for epidermal differentiation', Development, vol. 132, no. 5, pp. 1021-1034. https://doi.org/10.1242/dev.01641
Tao, Jianning ; Kuliyev, Emin ; Wang, Xi ; Li, Xiuling ; Wilanowski, Tomasz ; Jane, Stephen M. ; Mead, Paul E. ; Cunningham, John M. / BMP4-dependent expression of Xenopus Grainyhead-like 1 is essential for epidermal differentiation. In: Development. 2005 ; Vol. 132, No. 5. pp. 1021-1034.
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