Blood-borne macrophage-neural cell interactions hitchhike on endosome networks for cell-based nanozyme brain delivery

Matthew J. Haney, Poornima Suresh, Yuling Zhao, Georgette D Kanmogne, Irena Kadiu, Marina Sokolsky-Papkov, Natalia L. Klyachko, R Lee Mosley, Alexander V. Kabanov, Howard Eliot Gendelman, Elena V. Batrakova

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Background: Macrophage-carried nanoformulated catalase ('nanozyme) attenuates neuroinflammation and protects nigrostriatal neurons from 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine intoxication. This is facilitated by effective enzyme transfer from blood-borne macrophages to adjacent endothelial cells and neurons leading to the decomposition of reactive oxygen species. Materials & methods: We examined the intra- and inter-cellular trafficking mechanisms of nanozymes by confocal microscopy. Improved neuronal survival mediated by nanozyme-loaded macrophages was demonstrated by fluorescence-activated cell sorting. Results: In macrophages, nanozymes were internalized mainly by clathrin-mediated endocytosis then trafficked to recycling endosomes. The enzyme is subsequently released in exosomes facilitated by bridging conduits. Nanozyme transfer from macrophages to adjacent cells by endocytosis-independent mechanisms diffusing broadly throughout the recipient cells. In contrast, macrophage-free nanozymes were localized in lysosomes following endocytic entry. Conclusion: Facilitated transfer of nanozyme from cell to cell can improve neuroprotection against oxidative stress commonly seen during neurodegenerative disease processes. Original submitted 29 June 2011; Revised submitted 14 September 2011; Published online 11 January 201.

Original languageEnglish (US)
Pages (from-to)815-833
Number of pages19
JournalNanomedicine
Volume7
Issue number6
DOIs
StatePublished - Jun 1 2012

Fingerprint

Macrophages
Endosomes
Cell Communication
brain
Brain
Blood
blood
enzyme
trafficking
interaction
sorting
microscopy
fluorescence
recycling
intoxication
decomposition
Endocytosis
Neurons
Enzymes
recipient

Keywords

  • Parkinsons disease
  • blood-brain barrier
  • catalase
  • cell-mediated drug delivery
  • exosome
  • intracellular localization
  • macrophage
  • nanozyme

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)

Cite this

Blood-borne macrophage-neural cell interactions hitchhike on endosome networks for cell-based nanozyme brain delivery. / Haney, Matthew J.; Suresh, Poornima; Zhao, Yuling; Kanmogne, Georgette D; Kadiu, Irena; Sokolsky-Papkov, Marina; Klyachko, Natalia L.; Mosley, R Lee; Kabanov, Alexander V.; Gendelman, Howard Eliot; Batrakova, Elena V.

In: Nanomedicine, Vol. 7, No. 6, 01.06.2012, p. 815-833.

Research output: Contribution to journalArticle

Haney, MJ, Suresh, P, Zhao, Y, Kanmogne, GD, Kadiu, I, Sokolsky-Papkov, M, Klyachko, NL, Mosley, RL, Kabanov, AV, Gendelman, HE & Batrakova, EV 2012, 'Blood-borne macrophage-neural cell interactions hitchhike on endosome networks for cell-based nanozyme brain delivery', Nanomedicine, vol. 7, no. 6, pp. 815-833. https://doi.org/10.2217/nnm.11.156
Haney, Matthew J. ; Suresh, Poornima ; Zhao, Yuling ; Kanmogne, Georgette D ; Kadiu, Irena ; Sokolsky-Papkov, Marina ; Klyachko, Natalia L. ; Mosley, R Lee ; Kabanov, Alexander V. ; Gendelman, Howard Eliot ; Batrakova, Elena V. / Blood-borne macrophage-neural cell interactions hitchhike on endosome networks for cell-based nanozyme brain delivery. In: Nanomedicine. 2012 ; Vol. 7, No. 6. pp. 815-833.
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