Biotransformation of tetracycline by a novel bacterial strain Stenotrophomonas maltophilia DT1

Yifei Leng, Jianguo Bao, Gaofeng Chang, Han Zheng, Xingxing Li, Jiangkun Du, Daniel Snow, Xu Li

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Although several abiotic processes have been reported that can transform antibiotics, little is known about whether and how microbiological processes may degrade antibiotics in the environment. This work isolated one tetracycline degrading bacterial strain, Stenotrophomonas maltophilia strain DT1, and characterized the biotransformation of tetracycline by DT1 under various environmental conditions. The biotransformation rate was the highest when the initial pH was 9 and the reaction temperature was at 30 °C, and can be described using the Michaelis-Menten model under different initial tetracycline concentrations. When additional substrate was present, the substrate that caused increased biomass resulted in a decreased biotransformation rate of tetracycline. According to disk diffusion tests, the biotransformation products of tetracycline had lower antibiotic potency than the parent compound. Six possible biotransformation products were identified, and a potential biotransformation pathway was proposed that included sequential removal of N-methyl, carbonyl, and amine function groups. Results from this study can lead to better estimation of the fate and transport of antibiotics in the environment and has the potential to be utilized in designing engineering processes to remove tetracycline from water and soil.

Original languageEnglish (US)
Pages (from-to)125-133
Number of pages9
JournalJournal of Hazardous Materials
Volume318
DOIs
StatePublished - Nov 15 2016

Fingerprint

Stenotrophomonas maltophilia
biotransformation
Biotransformation
Tetracycline
Antibiotics
antibiotics
Anti-Bacterial Agents
Microbiological Phenomena
substrate
Substrates
Biomass
Amines
transform
Soil
environmental conditions
Soils
engineering
Temperature
Water
biomass

Keywords

  • Biotransformation
  • Hydrolysis
  • Stenotrophomonas maltophilia
  • Tetracycline
  • Transformation products

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution
  • Health, Toxicology and Mutagenesis

Cite this

Biotransformation of tetracycline by a novel bacterial strain Stenotrophomonas maltophilia DT1. / Leng, Yifei; Bao, Jianguo; Chang, Gaofeng; Zheng, Han; Li, Xingxing; Du, Jiangkun; Snow, Daniel; Li, Xu.

In: Journal of Hazardous Materials, Vol. 318, 15.11.2016, p. 125-133.

Research output: Contribution to journalArticle

Leng, Yifei ; Bao, Jianguo ; Chang, Gaofeng ; Zheng, Han ; Li, Xingxing ; Du, Jiangkun ; Snow, Daniel ; Li, Xu. / Biotransformation of tetracycline by a novel bacterial strain Stenotrophomonas maltophilia DT1. In: Journal of Hazardous Materials. 2016 ; Vol. 318. pp. 125-133.
@article{ed65944d876f42219575975f8b4a510b,
title = "Biotransformation of tetracycline by a novel bacterial strain Stenotrophomonas maltophilia DT1",
abstract = "Although several abiotic processes have been reported that can transform antibiotics, little is known about whether and how microbiological processes may degrade antibiotics in the environment. This work isolated one tetracycline degrading bacterial strain, Stenotrophomonas maltophilia strain DT1, and characterized the biotransformation of tetracycline by DT1 under various environmental conditions. The biotransformation rate was the highest when the initial pH was 9 and the reaction temperature was at 30 °C, and can be described using the Michaelis-Menten model under different initial tetracycline concentrations. When additional substrate was present, the substrate that caused increased biomass resulted in a decreased biotransformation rate of tetracycline. According to disk diffusion tests, the biotransformation products of tetracycline had lower antibiotic potency than the parent compound. Six possible biotransformation products were identified, and a potential biotransformation pathway was proposed that included sequential removal of N-methyl, carbonyl, and amine function groups. Results from this study can lead to better estimation of the fate and transport of antibiotics in the environment and has the potential to be utilized in designing engineering processes to remove tetracycline from water and soil.",
keywords = "Biotransformation, Hydrolysis, Stenotrophomonas maltophilia, Tetracycline, Transformation products",
author = "Yifei Leng and Jianguo Bao and Gaofeng Chang and Han Zheng and Xingxing Li and Jiangkun Du and Daniel Snow and Xu Li",
year = "2016",
month = "11",
day = "15",
doi = "10.1016/j.jhazmat.2016.06.053",
language = "English (US)",
volume = "318",
pages = "125--133",
journal = "Journal of Hazardous Materials",
issn = "0304-3894",
publisher = "Elsevier",

}

TY - JOUR

T1 - Biotransformation of tetracycline by a novel bacterial strain Stenotrophomonas maltophilia DT1

AU - Leng, Yifei

AU - Bao, Jianguo

AU - Chang, Gaofeng

AU - Zheng, Han

AU - Li, Xingxing

AU - Du, Jiangkun

AU - Snow, Daniel

AU - Li, Xu

PY - 2016/11/15

Y1 - 2016/11/15

N2 - Although several abiotic processes have been reported that can transform antibiotics, little is known about whether and how microbiological processes may degrade antibiotics in the environment. This work isolated one tetracycline degrading bacterial strain, Stenotrophomonas maltophilia strain DT1, and characterized the biotransformation of tetracycline by DT1 under various environmental conditions. The biotransformation rate was the highest when the initial pH was 9 and the reaction temperature was at 30 °C, and can be described using the Michaelis-Menten model under different initial tetracycline concentrations. When additional substrate was present, the substrate that caused increased biomass resulted in a decreased biotransformation rate of tetracycline. According to disk diffusion tests, the biotransformation products of tetracycline had lower antibiotic potency than the parent compound. Six possible biotransformation products were identified, and a potential biotransformation pathway was proposed that included sequential removal of N-methyl, carbonyl, and amine function groups. Results from this study can lead to better estimation of the fate and transport of antibiotics in the environment and has the potential to be utilized in designing engineering processes to remove tetracycline from water and soil.

AB - Although several abiotic processes have been reported that can transform antibiotics, little is known about whether and how microbiological processes may degrade antibiotics in the environment. This work isolated one tetracycline degrading bacterial strain, Stenotrophomonas maltophilia strain DT1, and characterized the biotransformation of tetracycline by DT1 under various environmental conditions. The biotransformation rate was the highest when the initial pH was 9 and the reaction temperature was at 30 °C, and can be described using the Michaelis-Menten model under different initial tetracycline concentrations. When additional substrate was present, the substrate that caused increased biomass resulted in a decreased biotransformation rate of tetracycline. According to disk diffusion tests, the biotransformation products of tetracycline had lower antibiotic potency than the parent compound. Six possible biotransformation products were identified, and a potential biotransformation pathway was proposed that included sequential removal of N-methyl, carbonyl, and amine function groups. Results from this study can lead to better estimation of the fate and transport of antibiotics in the environment and has the potential to be utilized in designing engineering processes to remove tetracycline from water and soil.

KW - Biotransformation

KW - Hydrolysis

KW - Stenotrophomonas maltophilia

KW - Tetracycline

KW - Transformation products

UR - http://www.scopus.com/inward/record.url?scp=84978180012&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84978180012&partnerID=8YFLogxK

U2 - 10.1016/j.jhazmat.2016.06.053

DO - 10.1016/j.jhazmat.2016.06.053

M3 - Article

C2 - 27420384

AN - SCOPUS:84978180012

VL - 318

SP - 125

EP - 133

JO - Journal of Hazardous Materials

JF - Journal of Hazardous Materials

SN - 0304-3894

ER -