Biotinylation of lysine 16 in histone H4 contributes toward nucleosome condensation

Mahendra P. Singh, Subhashinee S K Wijeratne, Janos Zempleni

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Holocarboxylase synthetase (HLCS) is part of a multiprotein gene repression complex and catalyzes the covalent binding of biotin to lysines (K) in histones H3 and H4, thereby creating rare gene repression marks such as K16-biotinylated histone H4 (H4K16bio). We tested the hypothesis that H4K16bio contributes toward nucleosome condensation and gene repression by HLCS. We used recombinant histone H4 in which K16 was mutated to a cysteine (H4K16C) for subsequent chemical biotinylation of the sulfhydryl group to create H4K16Cbio. Nucleosomes were assembled by using H4K16Cbio and the 'Widom 601' nucleosomal DNA position sequence; biotin-free histone H4 and H4K16C were used as controls. Nucleosomal compaction was analyzed using atomic force microscopy (AFM). The length of DNA per nucleosome was ∼30% greater in H4K16Cbio-containing histone octamers (61.14 ± 10.92 nm) compared with native H4 (46.89 ± 12.6 nm) and H4K16C (47.26 ± 10.32 nm), suggesting biotin-dependent chromatin condensation (P < 0.001). Likewise, the number of DNA turns around histone core octamers was ∼17.2% greater in in H4K16Cbio-containing octamers (1.78 ± 0.16) compared with native H4 (1.52 ± 0.21) and H4K16C (1.52 ± 0.17), judged by the rotation angle (P < 0.001; N = 150). We conclude that biotinylation of K16 in histone H4 contributes toward chromatin condensation.

Original languageEnglish (US)
Pages (from-to)105-111
Number of pages7
JournalArchives of Biochemistry and Biophysics
Volume529
Issue number2
DOIs
StatePublished - Jan 15 2013

Fingerprint

Biotinylation
Nucleosomes
Histones
Lysine
Condensation
Biotin
Genes
Chromatin
DNA
Atomic Force Microscopy
Cysteine
Atomic force microscopy
Compaction

Keywords

  • 'Widom 601' nucleosomal DNA position sequence
  • Atomic force microscopy
  • Biotin
  • Histone H4
  • Lysine-16
  • Nucleosomes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Biotinylation of lysine 16 in histone H4 contributes toward nucleosome condensation. / Singh, Mahendra P.; Wijeratne, Subhashinee S K; Zempleni, Janos.

In: Archives of Biochemistry and Biophysics, Vol. 529, No. 2, 15.01.2013, p. 105-111.

Research output: Contribution to journalArticle

Singh, Mahendra P. ; Wijeratne, Subhashinee S K ; Zempleni, Janos. / Biotinylation of lysine 16 in histone H4 contributes toward nucleosome condensation. In: Archives of Biochemistry and Biophysics. 2013 ; Vol. 529, No. 2. pp. 105-111.
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abstract = "Holocarboxylase synthetase (HLCS) is part of a multiprotein gene repression complex and catalyzes the covalent binding of biotin to lysines (K) in histones H3 and H4, thereby creating rare gene repression marks such as K16-biotinylated histone H4 (H4K16bio). We tested the hypothesis that H4K16bio contributes toward nucleosome condensation and gene repression by HLCS. We used recombinant histone H4 in which K16 was mutated to a cysteine (H4K16C) for subsequent chemical biotinylation of the sulfhydryl group to create H4K16Cbio. Nucleosomes were assembled by using H4K16Cbio and the 'Widom 601' nucleosomal DNA position sequence; biotin-free histone H4 and H4K16C were used as controls. Nucleosomal compaction was analyzed using atomic force microscopy (AFM). The length of DNA per nucleosome was ∼30{\%} greater in H4K16Cbio-containing histone octamers (61.14 ± 10.92 nm) compared with native H4 (46.89 ± 12.6 nm) and H4K16C (47.26 ± 10.32 nm), suggesting biotin-dependent chromatin condensation (P < 0.001). Likewise, the number of DNA turns around histone core octamers was ∼17.2{\%} greater in in H4K16Cbio-containing octamers (1.78 ± 0.16) compared with native H4 (1.52 ± 0.21) and H4K16C (1.52 ± 0.17), judged by the rotation angle (P < 0.001; N = 150). We conclude that biotinylation of K16 in histone H4 contributes toward chromatin condensation.",
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