Biotinylation of K12 in histone H4 decreases in response to DNA double-strand breaks in human JAr choriocarcinoma cells

Nagarama Kothapalli, Gautam Sarath, Janos Zempleni

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

We tested the hypothesis that biotinylation of K12 in histone H4 plays a role in the cellular response to double-strand breaks (DSB) of DNA in human cells. DSB were caused by treating choriocarcinoma JAr cells with etoposide. Biotinylation of K12 in histone H4 decreased by 50% as early as 10-20 min after initiation of treatment with etoposide. Biotinylation returned to initial levels 30-40 min after the addition of etoposide to the medium. Temporal patterns of K12-biotinylation were similar for human lymphoma cells. Phosphorylation of S14 of histone H2B and poly(ADP-ribosylation) of glutamate residues on histone H2A are known markers of DSB in DNA; these modifications increased 10-40 min after alterations in K12-biotinylation occurred. Decreased biotinylation of K12 of histone H4 was specific for DSB but was not detectable in response to single-strand breaks or the formation of thymine dimers. Biotin-deficient choriocarcinoma cells exhibited a 40% decrease in rates of survival in response to etoposide compared with biotin-sufficient controls. These studies suggest that the lack of biotinylation of K12 in histone H4 is an early signaling event in response to DSB.

Original languageEnglish (US)
Pages (from-to)2337-2342
Number of pages6
JournalJournal of Nutrition
Volume135
Issue number10
StatePublished - Oct 1 2005

Fingerprint

biotinylation
Biotinylation
Choriocarcinoma
Double-Stranded DNA Breaks
histones
Histones
Etoposide
DNA
cells
biotin
Biotin
Pyrimidine Dimers
thymine
lymphoma
glutamates
Adenosine Diphosphate
Glutamic Acid
Lymphoma
phosphorylation
survival rate

Keywords

  • Biotin
  • DNA damage
  • Double-strand breaks
  • Histone H4

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Biotinylation of K12 in histone H4 decreases in response to DNA double-strand breaks in human JAr choriocarcinoma cells. / Kothapalli, Nagarama; Sarath, Gautam; Zempleni, Janos.

In: Journal of Nutrition, Vol. 135, No. 10, 01.10.2005, p. 2337-2342.

Research output: Contribution to journalArticle

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