Biotinidase catalyzes debiotinylation of histones

Tyrie D. Ballard, Jon Wolff, Jacob B. Griffin, J. Steven Stanley, Sandy Van Calcar, Janos Zempleni

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Background Post-translational modifications of histones play important roles in processes such as regulation of gene expression and DNA repair. Recently, evidence has been provided that histones in human cells are modified by covalent attachment of biotin. Aim of the study: To determine whether the reverse process (debiotinylation of histones) occurs in biological samples and whether debiotinylation is an enzyme-mediated process; and to characterize the enzyme that mediates debiotinylation of histones. Methods: Plasma and lymphocytes from healthy adults and a biotinidase-deficient patient were used as sources of debiotinylating enzymes. Debiotinylation of histones by plasma and lymphocyte proteins was measured using a colorimetric 96-well plate assay. Results Histones were debiotinylated rapidly if incubated with human plasma or lysates of lymphocytes. The following observations are consistent with the hypothesis that debiotinylation is an enzyme-mediated process: (i) Hydrolysis was slower at 4 °C compared to 37 °C; (ii) debiotinylating activity was destroyed when biological samples were heated at 90 °C for 30 min preceding incubation with biotinylated histones; and (iii) rates of debiotinylation were pH dependent. Rates of histone debiotinylation were significantly decreased in biotinidase-deficient samples. Conclusion Debiotinylation of histones in human samples is an enzyme-mediated process that is at least partly catalyzed by biotinidase.

Original languageEnglish (US)
Pages (from-to)78-84
Number of pages7
JournalEuropean Journal of Nutrition
Volume41
Issue number2
DOIs
StatePublished - Dec 1 2002

Fingerprint

Biotinidase
histones
Histones
Enzymes
enzymes
lymphocytes
Lymphocytes
sampling
post-translational modification
gene expression regulation
Gene Expression Regulation
biotin
Post Translational Protein Processing
Biotin
DNA repair
DNA Repair
Blood Proteins
Hydrolysis
hydrolysis

Keywords

  • Biotin
  • Biotinidase
  • Biotinylation
  • Debiotinylation
  • Histone

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Ballard, T. D., Wolff, J., Griffin, J. B., Stanley, J. S., Van Calcar, S., & Zempleni, J. (2002). Biotinidase catalyzes debiotinylation of histones. European Journal of Nutrition, 41(2), 78-84. https://doi.org/10.1007/s003940200011

Biotinidase catalyzes debiotinylation of histones. / Ballard, Tyrie D.; Wolff, Jon; Griffin, Jacob B.; Stanley, J. Steven; Van Calcar, Sandy; Zempleni, Janos.

In: European Journal of Nutrition, Vol. 41, No. 2, 01.12.2002, p. 78-84.

Research output: Contribution to journalArticle

Ballard, TD, Wolff, J, Griffin, JB, Stanley, JS, Van Calcar, S & Zempleni, J 2002, 'Biotinidase catalyzes debiotinylation of histones', European Journal of Nutrition, vol. 41, no. 2, pp. 78-84. https://doi.org/10.1007/s003940200011
Ballard TD, Wolff J, Griffin JB, Stanley JS, Van Calcar S, Zempleni J. Biotinidase catalyzes debiotinylation of histones. European Journal of Nutrition. 2002 Dec 1;41(2):78-84. https://doi.org/10.1007/s003940200011
Ballard, Tyrie D. ; Wolff, Jon ; Griffin, Jacob B. ; Stanley, J. Steven ; Van Calcar, Sandy ; Zempleni, Janos. / Biotinidase catalyzes debiotinylation of histones. In: European Journal of Nutrition. 2002 ; Vol. 41, No. 2. pp. 78-84.
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