Biotin supplementation increases expression of the cytochrome P 450 1B1 gene in Jurkat cells, increasing the occurrence of single-stranded DNA breaks

Rocio Rodriguez-Melendez, Jacob B. Griffin, Janos Zempleni

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

DNA microarray studies provided evidence that biotin supplementation increases the abundance of mRNA encoding cytochrome P450 1B1 (CYP1B1) in human lymphocytes. CYP1B1 hydroxylates procarcinogens, generating electrophilic mutagens. Here, we sought to identify the signaling pathways that increase the expression of CYP1B1 in biotin-supplemented human T (Jurkat) cells and to determine whether activation of the CYP1B1 gene is associated with increased occurrence of single-stranded DNA breaks. Jurkat cells were cultured in biotin-deficient (0.025 nmol/L) and biotin-supplemented (10 nmol/L) media. The transcriptional activity of a CYP1B1 reporter gene construct was 24% greater in biotin-supplemented compared with biotin-deficient cells (P < 0.01). Similarly, the abundance of CYP1B1 mRNA was 72% greater in biotin-supplemented than in biotin-deficient cells (P < 0.05). Electrophoretic mobility shift assays suggested that Sp1 sites in the regulatory region of the CYP1B1 gene play important roles in transcriptional activation by biotin. The abundance of CYP1B1 protein and activity of CYP1B1 were 124 and 35% greater, respectively, in biotin-supplemented compared with biotin-deficient cells (P < 0.05). The increased expression of CYP1B1 in biotin-supplemented cells was associated with an increase in the occurrence of single-stranded DNA breaks compared with biotin-deficient cells; synthetic inhibitors of CYP1B1 prevented strand breaks, suggesting that the effects of biotin were specific for CYP1B1. These studies provide evidence that transcription factors with an affinity for Sp1 sites mediate transcriptional activation of the CYP1B1 gene in biotin-supplemented T cells, increasing the occurrence of single-stranded DNA breaks.

Original languageEnglish (US)
Pages (from-to)2222-2228
Number of pages7
JournalJournal of Nutrition
Volume134
Issue number9
StatePublished - Sep 1 2004

Fingerprint

Single-Stranded DNA Breaks
single-stranded DNA
Jurkat Cells
biotin
Biotin
cytochrome P-450
Cytochrome P-450 Enzyme System
Genes
genes
cells
transcriptional activation
Transcriptional Activation
T-lymphocytes
Messenger RNA
Nucleic Acid Regulatory Sequences
Mutagens
Electrophoretic Mobility Shift Assay
Oligonucleotide Array Sequence Analysis
Reporter Genes

Keywords

  • Biotin
  • Cytochrome P 1B1
  • DNA breaks
  • Human
  • Jurkat cells

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Biotin supplementation increases expression of the cytochrome P 450 1B1 gene in Jurkat cells, increasing the occurrence of single-stranded DNA breaks. / Rodriguez-Melendez, Rocio; Griffin, Jacob B.; Zempleni, Janos.

In: Journal of Nutrition, Vol. 134, No. 9, 01.09.2004, p. 2222-2228.

Research output: Contribution to journalArticle

@article{5c5935b65cd44a36a2956480cf7a7a12,
title = "Biotin supplementation increases expression of the cytochrome P 450 1B1 gene in Jurkat cells, increasing the occurrence of single-stranded DNA breaks",
abstract = "DNA microarray studies provided evidence that biotin supplementation increases the abundance of mRNA encoding cytochrome P450 1B1 (CYP1B1) in human lymphocytes. CYP1B1 hydroxylates procarcinogens, generating electrophilic mutagens. Here, we sought to identify the signaling pathways that increase the expression of CYP1B1 in biotin-supplemented human T (Jurkat) cells and to determine whether activation of the CYP1B1 gene is associated with increased occurrence of single-stranded DNA breaks. Jurkat cells were cultured in biotin-deficient (0.025 nmol/L) and biotin-supplemented (10 nmol/L) media. The transcriptional activity of a CYP1B1 reporter gene construct was 24{\%} greater in biotin-supplemented compared with biotin-deficient cells (P < 0.01). Similarly, the abundance of CYP1B1 mRNA was 72{\%} greater in biotin-supplemented than in biotin-deficient cells (P < 0.05). Electrophoretic mobility shift assays suggested that Sp1 sites in the regulatory region of the CYP1B1 gene play important roles in transcriptional activation by biotin. The abundance of CYP1B1 protein and activity of CYP1B1 were 124 and 35{\%} greater, respectively, in biotin-supplemented compared with biotin-deficient cells (P < 0.05). The increased expression of CYP1B1 in biotin-supplemented cells was associated with an increase in the occurrence of single-stranded DNA breaks compared with biotin-deficient cells; synthetic inhibitors of CYP1B1 prevented strand breaks, suggesting that the effects of biotin were specific for CYP1B1. These studies provide evidence that transcription factors with an affinity for Sp1 sites mediate transcriptional activation of the CYP1B1 gene in biotin-supplemented T cells, increasing the occurrence of single-stranded DNA breaks.",
keywords = "Biotin, Cytochrome P 1B1, DNA breaks, Human, Jurkat cells",
author = "Rocio Rodriguez-Melendez and Griffin, {Jacob B.} and Janos Zempleni",
year = "2004",
month = "9",
day = "1",
language = "English (US)",
volume = "134",
pages = "2222--2228",
journal = "The Journal of nutrition",
issn = "0022-3166",
publisher = "American Society for Nutrition",
number = "9",

}

TY - JOUR

T1 - Biotin supplementation increases expression of the cytochrome P 450 1B1 gene in Jurkat cells, increasing the occurrence of single-stranded DNA breaks

AU - Rodriguez-Melendez, Rocio

AU - Griffin, Jacob B.

AU - Zempleni, Janos

PY - 2004/9/1

Y1 - 2004/9/1

N2 - DNA microarray studies provided evidence that biotin supplementation increases the abundance of mRNA encoding cytochrome P450 1B1 (CYP1B1) in human lymphocytes. CYP1B1 hydroxylates procarcinogens, generating electrophilic mutagens. Here, we sought to identify the signaling pathways that increase the expression of CYP1B1 in biotin-supplemented human T (Jurkat) cells and to determine whether activation of the CYP1B1 gene is associated with increased occurrence of single-stranded DNA breaks. Jurkat cells were cultured in biotin-deficient (0.025 nmol/L) and biotin-supplemented (10 nmol/L) media. The transcriptional activity of a CYP1B1 reporter gene construct was 24% greater in biotin-supplemented compared with biotin-deficient cells (P < 0.01). Similarly, the abundance of CYP1B1 mRNA was 72% greater in biotin-supplemented than in biotin-deficient cells (P < 0.05). Electrophoretic mobility shift assays suggested that Sp1 sites in the regulatory region of the CYP1B1 gene play important roles in transcriptional activation by biotin. The abundance of CYP1B1 protein and activity of CYP1B1 were 124 and 35% greater, respectively, in biotin-supplemented compared with biotin-deficient cells (P < 0.05). The increased expression of CYP1B1 in biotin-supplemented cells was associated with an increase in the occurrence of single-stranded DNA breaks compared with biotin-deficient cells; synthetic inhibitors of CYP1B1 prevented strand breaks, suggesting that the effects of biotin were specific for CYP1B1. These studies provide evidence that transcription factors with an affinity for Sp1 sites mediate transcriptional activation of the CYP1B1 gene in biotin-supplemented T cells, increasing the occurrence of single-stranded DNA breaks.

AB - DNA microarray studies provided evidence that biotin supplementation increases the abundance of mRNA encoding cytochrome P450 1B1 (CYP1B1) in human lymphocytes. CYP1B1 hydroxylates procarcinogens, generating electrophilic mutagens. Here, we sought to identify the signaling pathways that increase the expression of CYP1B1 in biotin-supplemented human T (Jurkat) cells and to determine whether activation of the CYP1B1 gene is associated with increased occurrence of single-stranded DNA breaks. Jurkat cells were cultured in biotin-deficient (0.025 nmol/L) and biotin-supplemented (10 nmol/L) media. The transcriptional activity of a CYP1B1 reporter gene construct was 24% greater in biotin-supplemented compared with biotin-deficient cells (P < 0.01). Similarly, the abundance of CYP1B1 mRNA was 72% greater in biotin-supplemented than in biotin-deficient cells (P < 0.05). Electrophoretic mobility shift assays suggested that Sp1 sites in the regulatory region of the CYP1B1 gene play important roles in transcriptional activation by biotin. The abundance of CYP1B1 protein and activity of CYP1B1 were 124 and 35% greater, respectively, in biotin-supplemented compared with biotin-deficient cells (P < 0.05). The increased expression of CYP1B1 in biotin-supplemented cells was associated with an increase in the occurrence of single-stranded DNA breaks compared with biotin-deficient cells; synthetic inhibitors of CYP1B1 prevented strand breaks, suggesting that the effects of biotin were specific for CYP1B1. These studies provide evidence that transcription factors with an affinity for Sp1 sites mediate transcriptional activation of the CYP1B1 gene in biotin-supplemented T cells, increasing the occurrence of single-stranded DNA breaks.

KW - Biotin

KW - Cytochrome P 1B1

KW - DNA breaks

KW - Human

KW - Jurkat cells

UR - http://www.scopus.com/inward/record.url?scp=4444240734&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4444240734&partnerID=8YFLogxK

M3 - Article

C2 - 15333708

AN - SCOPUS:4444240734

VL - 134

SP - 2222

EP - 2228

JO - The Journal of nutrition

JF - The Journal of nutrition

SN - 0022-3166

IS - 9

ER -