Biotechnological production of 1,2,4-butanetriol: An efficient process to synthesize energetic material precursor from renewable biomass

Yujin Cao, Wei Niu, Jiantao Guo, Mo Xian, Huizhou Liu

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

1,2,4-Butanetriol (BT) is a valuable chemical with extensive applications in many different fields. The traditional chemical routes to synthesize BT suffer from many drawbacks, e.g., harsh reaction conditions, multiple steps and poor selectivity, limiting its industrial production. In this study, an engineered Escherichia coli strain was constructed to produce BT from xylose, which is a major component of the lignocellulosic biomass. Through the coexpression of a xylose dehydrogenase (CCxylB) and a xylonolactonase (xylC) from Caulobacter crescentus, native E. coli xylonate dehydratase (yjhG), a 2-keto acid decarboxylase from Pseudomonas putida (mdlC) and native E. coli aldehyde reductase (adhP) in E. coli BL21 star(DE3), the recombinant strain could efficiently convert xylose to BT. Furthermore, the competitive pathway responsible for xylose metabolism in E. coli was blocked by disrupting two genes (xylA and EcxylB) encoding xylose isomerase and xyloluse kinase. Under fed-batch conditions, the engineered strain BL21δxylAB/pE-mdlCxylBCandpA-adhPyjhG produced up to 3.92 g/L of BT from 20 g/L of xylose, corresponding to a molar yield of 27.7%. These results suggest that the engineered E. coli has a promising prospect for the large-scale production of BT.

Original languageEnglish (US)
Article number18149
JournalScientific reports
Volume5
DOIs
StatePublished - Dec 16 2015

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Xylose
Biomass
Escherichia coli
xylose isomerase
Caulobacter crescentus
Hydro-Lyases
Keto Acids
Aldehyde Reductase
Pseudomonas putida
Carboxy-Lyases
1,2,4-butanetriol
Oxidoreductases
Phosphotransferases
Genes

ASJC Scopus subject areas

  • General

Cite this

Biotechnological production of 1,2,4-butanetriol : An efficient process to synthesize energetic material precursor from renewable biomass. / Cao, Yujin; Niu, Wei; Guo, Jiantao; Xian, Mo; Liu, Huizhou.

In: Scientific reports, Vol. 5, 18149, 16.12.2015.

Research output: Contribution to journalArticle

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abstract = "1,2,4-Butanetriol (BT) is a valuable chemical with extensive applications in many different fields. The traditional chemical routes to synthesize BT suffer from many drawbacks, e.g., harsh reaction conditions, multiple steps and poor selectivity, limiting its industrial production. In this study, an engineered Escherichia coli strain was constructed to produce BT from xylose, which is a major component of the lignocellulosic biomass. Through the coexpression of a xylose dehydrogenase (CCxylB) and a xylonolactonase (xylC) from Caulobacter crescentus, native E. coli xylonate dehydratase (yjhG), a 2-keto acid decarboxylase from Pseudomonas putida (mdlC) and native E. coli aldehyde reductase (adhP) in E. coli BL21 star(DE3), the recombinant strain could efficiently convert xylose to BT. Furthermore, the competitive pathway responsible for xylose metabolism in E. coli was blocked by disrupting two genes (xylA and EcxylB) encoding xylose isomerase and xyloluse kinase. Under fed-batch conditions, the engineered strain BL21δxylAB/pE-mdlCxylBCandpA-adhPyjhG produced up to 3.92 g/L of BT from 20 g/L of xylose, corresponding to a molar yield of 27.7{\%}. These results suggest that the engineered E. coli has a promising prospect for the large-scale production of BT.",
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