Biosynthesis of wyosine derivatives in tRNA: An ancient and highly diverse pathway in archaea

Valérie De Crécy-Lagard, Céline Brochier-Armanet, Jaunius Urbonavicius, Bernard Fernandez, Gabriela Phillips, Benjamin Lyons, Akiko Noma, Sophie Alvarez, Louis Droogmans, Jean Armengaud, Henri Grosjean

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

Wyosine (imG) and its derivatives such as wybutosine (yW) are found at position 37 of phenylalanine-specific transfer RNA (tRNAPhe), 3′ adjacent to the anticodon in Eucarya and Archaea. In Saccharomyces cerevisiae, formation of yW requires five enzymes acting in a strictly sequential order: Trm5, Tyw1, Tyw2, Tyw3, and Tyw4. Archaea contain wyosine derivatives, but their diversity is greater than in eukaryotes and the corresponding biosynthesis pathways still unknown. To identify these pathways, we analyzed the phylogenetic distribution of homologues of the yeast wybutosine biosynthesis proteins in 62 archaeal genomes and proposed a scenario for the origin and evolution of wyosine derivatives biosynthesis in Archaea that was partly experimentally validated. The key observations were 1) that four of the five wybutosine biosynthetic enzymes are ancient and may have been present in the last common ancestor of Archaea and Eucarya, 2) that the variations in the distribution pattern of biosynthesis enzymes reflect the diversity of the wyosine derivatives found in different Archaea. We also identified 7-aminocarboxypropyl-demethylwyosine (yW-86) and its N4-methyl derivative (yW-72) as final products in tRNAs of several Archaea when these were previously thought to be only intermediates of the eukaryotic pathway. We confirmed that isowyosine (imG2) and 7-methylwyosine (mimG) are two archaeal-specific guanosine-37 derivatives found in tRNA of both Euryarchaeota and Crenarchaeota. Finally, we proposed that the duplication of the trm5 gene in some Archaea led to a change in function from N1 methylation of guanosine to C7 methylation of 4-demethylwyosine (imG-14).

Original languageEnglish (US)
Pages (from-to)2062-2077
Number of pages16
JournalMolecular biology and evolution
Volume27
Issue number9
DOIs
StatePublished - Oct 1 2010

Fingerprint

Archaea
Transfer RNA
biosynthesis
methylation
chemical derivatives
enzyme
Eukaryota
guanosine
Guanosine
common ancestry
eukaryote
Methylation
Archaeal Genome
Enzymes
Crenarchaeota
yeast
enzymes
RNA
Euryarchaeota
RNA, Transfer, Phe

Keywords

  • Archaea
  • anticodon loop
  • biosynthetic pathway
  • evolution
  • mass spectrometry
  • methyltransferase
  • modification enzymes
  • phylogeny
  • tRNA

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

Cite this

De Crécy-Lagard, V., Brochier-Armanet, C., Urbonavicius, J., Fernandez, B., Phillips, G., Lyons, B., ... Grosjean, H. (2010). Biosynthesis of wyosine derivatives in tRNA: An ancient and highly diverse pathway in archaea. Molecular biology and evolution, 27(9), 2062-2077. https://doi.org/10.1093/molbev/msq096

Biosynthesis of wyosine derivatives in tRNA : An ancient and highly diverse pathway in archaea. / De Crécy-Lagard, Valérie; Brochier-Armanet, Céline; Urbonavicius, Jaunius; Fernandez, Bernard; Phillips, Gabriela; Lyons, Benjamin; Noma, Akiko; Alvarez, Sophie; Droogmans, Louis; Armengaud, Jean; Grosjean, Henri.

In: Molecular biology and evolution, Vol. 27, No. 9, 01.10.2010, p. 2062-2077.

Research output: Contribution to journalArticle

De Crécy-Lagard, V, Brochier-Armanet, C, Urbonavicius, J, Fernandez, B, Phillips, G, Lyons, B, Noma, A, Alvarez, S, Droogmans, L, Armengaud, J & Grosjean, H 2010, 'Biosynthesis of wyosine derivatives in tRNA: An ancient and highly diverse pathway in archaea', Molecular biology and evolution, vol. 27, no. 9, pp. 2062-2077. https://doi.org/10.1093/molbev/msq096
De Crécy-Lagard V, Brochier-Armanet C, Urbonavicius J, Fernandez B, Phillips G, Lyons B et al. Biosynthesis of wyosine derivatives in tRNA: An ancient and highly diverse pathway in archaea. Molecular biology and evolution. 2010 Oct 1;27(9):2062-2077. https://doi.org/10.1093/molbev/msq096
De Crécy-Lagard, Valérie ; Brochier-Armanet, Céline ; Urbonavicius, Jaunius ; Fernandez, Bernard ; Phillips, Gabriela ; Lyons, Benjamin ; Noma, Akiko ; Alvarez, Sophie ; Droogmans, Louis ; Armengaud, Jean ; Grosjean, Henri. / Biosynthesis of wyosine derivatives in tRNA : An ancient and highly diverse pathway in archaea. In: Molecular biology and evolution. 2010 ; Vol. 27, No. 9. pp. 2062-2077.
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