Bioresponsive hyperbranched polymers for siRNA and miRNA delivery

Ulrik L. Rahbek, Anne F. Nielsen, Mingdong Dong, Yezi You, Anne Chauchereau, David Oupicky, Flemming Besenbacher, Jørgen Kjems, Kenneth A. Howard

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

This work presents the novel use of reducible hyperbranched (rHB) polymers for delivery of RNA interference (RNAi) therapeutics. Cationic poly(amido amine) hyperbranched polymers that contain different contents of reducible disulfide to nonreducible linkages (0%, 17%, 25%, and 50%) were used to form interpolyelectrolyte polyplexes with siRNA and precursor miRNA (pre-miRNA). Atomic force microscopy (AFM) revealed rHB complexes of ∼100nm in size, which exhibited redox-activated disassembly in the presence of dithiothreitol (DTT). The complexes were avidly internalized and showed no cellular toxicity in an endogenous enhanced green fluorescence protein (EGFP) expressing H1299 human lung cancer cell line. The highest specific EGFP gene silencing (∼75%) was achieved with rHB (17%)/siRNA complexes at a weight-to-weight (w/w) ratio of 40 that correlated with the ability for this polymer to successfully transfect pre-miRNA. Evaluation of temporal silencing levels over 72h revealed incremental knockdown that reached a maximum at 72h for the rHB (50%) complexes, in contrast to maximum knockdown at 24h that remained relatively consistent, thereafter, for the rHB (17%), rHB (25%), and non-rHB complexes. The role of particle disassembly for intracellular targeting and modulation of gene silencing addressed in this work are important considerations in the development of this and other next-generation delivery systems.

Original languageEnglish (US)
Pages (from-to)812-820
Number of pages9
JournalJournal of Drug Targeting
Volume18
Issue number10
DOIs
StatePublished - Dec 1 2010

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Keywords

  • Hyperbranched
  • Poly(amido amine)
  • Pre-miRNA
  • RNA interference
  • Reducible
  • SiRNA

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Rahbek, U. L., Nielsen, A. F., Dong, M., You, Y., Chauchereau, A., Oupicky, D., Besenbacher, F., Kjems, J., & Howard, K. A. (2010). Bioresponsive hyperbranched polymers for siRNA and miRNA delivery. Journal of Drug Targeting, 18(10), 812-820. https://doi.org/10.3109/1061186X.2010.527982