Bioprinted Osteogenic and Vasculogenic Patterns for Engineering 3D Bone Tissue

Batzaya Byambaa, Nasim Annabi, Kan Yue, Grissel Trujillo-de Santiago, Mario Moisés Alvarez, Weitao Jia, Mehdi Kazemzadeh-Narbat, Su Ryon Shin, Ali Tamayol, Ali Khademhosseini

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Fabricating 3D large-scale bone tissue constructs with functional vasculature has been a particular challenge in engineering tissues suitable for repairing large bone defects. To address this challenge, an extrusion-based direct-writing bioprinting strategy is utilized to fabricate microstructured bone-like tissue constructs containing a perfusable vascular lumen. The bioprinted constructs are used as biomimetic in vitro matrices to co-culture human umbilical vein endothelial cells and bone marrow derived human mesenchymal stem cells in a naturally derived hydrogel. To form the perfusable blood vessel inside the bioprinted construct, a central cylinder with 5% gelatin methacryloyl (GelMA) hydrogel at low methacryloyl substitution (GelMALOW) was printed. We also develop cell-laden cylinder elements made of GelMA hydrogel loaded with silicate nanoplatelets to induce osteogenesis, and synthesized hydrogel formulations with chemically conjugated vascular endothelial growth factor to promote vascular spreading. It was found that the engineered construct is able to support cell survival and proliferation during maturation in vitro. Additionally, the whole construct demonstrates high structural stability during the in vitro culture for 21 days. This method enables the local control of physical and chemical microniches and the establishment of gradients in the bioprinted constructs.

Original languageEnglish (US)
Article number1700015
JournalAdvanced Healthcare Materials
Volume6
Issue number16
DOIs
StatePublished - Aug 23 2017

Fingerprint

Hydrogel
Hydrogels
Bone
Tissue
Blood Vessels
Bone and Bones
Gelatin
Bioprinting
Silicates
Biomimetics
Endothelial cells
Human Umbilical Vein Endothelial Cells
Blood vessels
Tissue Engineering
Coculture Techniques
Stem cells
Mesenchymal Stromal Cells
Tissue engineering
Cell culture
Osteogenesis

Keywords

  • 3D bioprinting
  • angiogenic hydrogels
  • bone-like tissue constructs
  • vascularized bone tissue

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Pharmaceutical Science

Cite this

Byambaa, B., Annabi, N., Yue, K., Trujillo-de Santiago, G., Alvarez, M. M., Jia, W., ... Khademhosseini, A. (2017). Bioprinted Osteogenic and Vasculogenic Patterns for Engineering 3D Bone Tissue. Advanced Healthcare Materials, 6(16), [1700015]. https://doi.org/10.1002/adhm.201700015

Bioprinted Osteogenic and Vasculogenic Patterns for Engineering 3D Bone Tissue. / Byambaa, Batzaya; Annabi, Nasim; Yue, Kan; Trujillo-de Santiago, Grissel; Alvarez, Mario Moisés; Jia, Weitao; Kazemzadeh-Narbat, Mehdi; Shin, Su Ryon; Tamayol, Ali; Khademhosseini, Ali.

In: Advanced Healthcare Materials, Vol. 6, No. 16, 1700015, 23.08.2017.

Research output: Contribution to journalArticle

Byambaa, B, Annabi, N, Yue, K, Trujillo-de Santiago, G, Alvarez, MM, Jia, W, Kazemzadeh-Narbat, M, Shin, SR, Tamayol, A & Khademhosseini, A 2017, 'Bioprinted Osteogenic and Vasculogenic Patterns for Engineering 3D Bone Tissue', Advanced Healthcare Materials, vol. 6, no. 16, 1700015. https://doi.org/10.1002/adhm.201700015
Byambaa B, Annabi N, Yue K, Trujillo-de Santiago G, Alvarez MM, Jia W et al. Bioprinted Osteogenic and Vasculogenic Patterns for Engineering 3D Bone Tissue. Advanced Healthcare Materials. 2017 Aug 23;6(16). 1700015. https://doi.org/10.1002/adhm.201700015
Byambaa, Batzaya ; Annabi, Nasim ; Yue, Kan ; Trujillo-de Santiago, Grissel ; Alvarez, Mario Moisés ; Jia, Weitao ; Kazemzadeh-Narbat, Mehdi ; Shin, Su Ryon ; Tamayol, Ali ; Khademhosseini, Ali. / Bioprinted Osteogenic and Vasculogenic Patterns for Engineering 3D Bone Tissue. In: Advanced Healthcare Materials. 2017 ; Vol. 6, No. 16.
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