Biomarkers of cellular apoptosis and necrosis in donor myocardium are not predictive of primary graft dysfunction

O. Szarszoi, J. Besik, M. Smetana, J. Maly, Marian Urban, J. Maluskova, A. Lodererova, L. Hoskova, Z. Tucanova, J. Pirk, I. Netuka

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Primary graft dysfunction (PGD) is a life-threatening complication among heart transplant recipients and a major cause of early mortality. Although the pathogenesis of PGD is still unclear, ischemia/reperfusion injury has been identified as a predominant factor. Both necrosis and apoptosis contribute to the loss of cardiomyocytes during ischemia/reperfusion injury, and this loss of cells can ultimately lead to PGD. The aim of our prospective study was to find out whether cell death, necrosis and apoptosis markers present in the donor myocardium can predict PGD. The prospective study involved 64 consecutive patients who underwent orthotopic heart transplantation at our institute between September 2010 and January 2013. High-sensitive cardiac troponin T (hs-cTnT) as a marker of minor myocardial necrosis was detected from arterial blood samples before the donor's pericardium was opened. Apoptosis (caspase-3, active + pro-caspase-3, bcl-2, TUNEL) was assessed from bioptic samples taken from the right ventricle prior graft harvesting. In our study, 14 % of transplant recipients developed PGD classified according to the standardized definition proposed by the ISHLT Working Group. We did not find differences between the groups in regard to hs-cTnT serum levels. The mean hs-cTnT value for the PGD group was 57.4±22.9 ng/l, compared to 68.4±10.8 ng/l in the group without PGD. The presence and severity of apoptosis in grafted hearts did not differ between grafts without PGD and hearts that subsequently developed PGD. In conclusion, our findings did not demonstrate any association between measured myocardial cell death, necrosis or apoptosis markers in donor myocardium and PGD in allograft recipients. More detailed investigations of cell death signaling pathways in transplanted hearts are required.

Original languageEnglish (US)
Pages (from-to)251-257
Number of pages7
JournalPhysiological Research
Volume65
Issue number2
StatePublished - Jan 1 2016
Externally publishedYes

Fingerprint

Primary Graft Dysfunction
Myocardium
Necrosis
Biomarkers
Tissue Donors
Apoptosis
Troponin T
Cell Death
Reperfusion Injury
Caspase 3
Prospective Studies
Transplants
Pericardium
In Situ Nick-End Labeling
Heart Transplantation
Cardiac Myocytes
Heart Ventricles
Allografts

Keywords

  • Apoptosis
  • Heart transplantation
  • Necrosis
  • Primary graft dysfunction

ASJC Scopus subject areas

  • Medicine(all)
  • Physiology

Cite this

Szarszoi, O., Besik, J., Smetana, M., Maly, J., Urban, M., Maluskova, J., ... Netuka, I. (2016). Biomarkers of cellular apoptosis and necrosis in donor myocardium are not predictive of primary graft dysfunction. Physiological Research, 65(2), 251-257.

Biomarkers of cellular apoptosis and necrosis in donor myocardium are not predictive of primary graft dysfunction. / Szarszoi, O.; Besik, J.; Smetana, M.; Maly, J.; Urban, Marian; Maluskova, J.; Lodererova, A.; Hoskova, L.; Tucanova, Z.; Pirk, J.; Netuka, I.

In: Physiological Research, Vol. 65, No. 2, 01.01.2016, p. 251-257.

Research output: Contribution to journalArticle

Szarszoi, O, Besik, J, Smetana, M, Maly, J, Urban, M, Maluskova, J, Lodererova, A, Hoskova, L, Tucanova, Z, Pirk, J & Netuka, I 2016, 'Biomarkers of cellular apoptosis and necrosis in donor myocardium are not predictive of primary graft dysfunction', Physiological Research, vol. 65, no. 2, pp. 251-257.
Szarszoi, O. ; Besik, J. ; Smetana, M. ; Maly, J. ; Urban, Marian ; Maluskova, J. ; Lodererova, A. ; Hoskova, L. ; Tucanova, Z. ; Pirk, J. ; Netuka, I. / Biomarkers of cellular apoptosis and necrosis in donor myocardium are not predictive of primary graft dysfunction. In: Physiological Research. 2016 ; Vol. 65, No. 2. pp. 251-257.
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abstract = "Primary graft dysfunction (PGD) is a life-threatening complication among heart transplant recipients and a major cause of early mortality. Although the pathogenesis of PGD is still unclear, ischemia/reperfusion injury has been identified as a predominant factor. Both necrosis and apoptosis contribute to the loss of cardiomyocytes during ischemia/reperfusion injury, and this loss of cells can ultimately lead to PGD. The aim of our prospective study was to find out whether cell death, necrosis and apoptosis markers present in the donor myocardium can predict PGD. The prospective study involved 64 consecutive patients who underwent orthotopic heart transplantation at our institute between September 2010 and January 2013. High-sensitive cardiac troponin T (hs-cTnT) as a marker of minor myocardial necrosis was detected from arterial blood samples before the donor's pericardium was opened. Apoptosis (caspase-3, active + pro-caspase-3, bcl-2, TUNEL) was assessed from bioptic samples taken from the right ventricle prior graft harvesting. In our study, 14 {\%} of transplant recipients developed PGD classified according to the standardized definition proposed by the ISHLT Working Group. We did not find differences between the groups in regard to hs-cTnT serum levels. The mean hs-cTnT value for the PGD group was 57.4±22.9 ng/l, compared to 68.4±10.8 ng/l in the group without PGD. The presence and severity of apoptosis in grafted hearts did not differ between grafts without PGD and hearts that subsequently developed PGD. In conclusion, our findings did not demonstrate any association between measured myocardial cell death, necrosis or apoptosis markers in donor myocardium and PGD in allograft recipients. More detailed investigations of cell death signaling pathways in transplanted hearts are required.",
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AU - Besik, J.

AU - Smetana, M.

AU - Maly, J.

AU - Urban, Marian

AU - Maluskova, J.

AU - Lodererova, A.

AU - Hoskova, L.

AU - Tucanova, Z.

AU - Pirk, J.

AU - Netuka, I.

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AB - Primary graft dysfunction (PGD) is a life-threatening complication among heart transplant recipients and a major cause of early mortality. Although the pathogenesis of PGD is still unclear, ischemia/reperfusion injury has been identified as a predominant factor. Both necrosis and apoptosis contribute to the loss of cardiomyocytes during ischemia/reperfusion injury, and this loss of cells can ultimately lead to PGD. The aim of our prospective study was to find out whether cell death, necrosis and apoptosis markers present in the donor myocardium can predict PGD. The prospective study involved 64 consecutive patients who underwent orthotopic heart transplantation at our institute between September 2010 and January 2013. High-sensitive cardiac troponin T (hs-cTnT) as a marker of minor myocardial necrosis was detected from arterial blood samples before the donor's pericardium was opened. Apoptosis (caspase-3, active + pro-caspase-3, bcl-2, TUNEL) was assessed from bioptic samples taken from the right ventricle prior graft harvesting. In our study, 14 % of transplant recipients developed PGD classified according to the standardized definition proposed by the ISHLT Working Group. We did not find differences between the groups in regard to hs-cTnT serum levels. The mean hs-cTnT value for the PGD group was 57.4±22.9 ng/l, compared to 68.4±10.8 ng/l in the group without PGD. The presence and severity of apoptosis in grafted hearts did not differ between grafts without PGD and hearts that subsequently developed PGD. In conclusion, our findings did not demonstrate any association between measured myocardial cell death, necrosis or apoptosis markers in donor myocardium and PGD in allograft recipients. More detailed investigations of cell death signaling pathways in transplanted hearts are required.

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