Biologically inspired sensing

Infrared spectroscopic analysis of cell responses to an inhalation health hazard

Mark Riley, Diana DeRosa, Jeanette Blaine, B. G. Potter, Pierre Lucas, David Le Coq, Christophe Juncker, Dianne E. Boesewetter, Jayne M. Collier, Catherine Boussard-Plédel, Bruno Bureau

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

This work describes the development of a biologically based sensing technique to quantify chemical agents that pose inhalation health hazards. The approach utilizes cultured epithelial cells (A549 human type II pneumocytes) of the lung, exposed to potential toxins and monitored through the noninvasive means of infrared spectroscopy to quantify changes to cell physiology and function. Cell response to Streptolysin O, a cholesterol-binding cytolysin, is investigated here. Infrared spectra display changes in cell physiology indicative of membrane damage, altered proteins, and some nucleic acid damage. Methods to improve cell adhesion through modification of support surface properties are detailed. This spectroscopic approach not only provides a robust means to detect potential toxins but also provides information on modes of damage and mechanisms of cellular response.

Original languageEnglish (US)
Pages (from-to)24-31
Number of pages8
JournalBiotechnology Progress
Volume22
Issue number1
DOIs
StatePublished - Jan 1 2006

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Cell Physiological Phenomena
Inhalation
Alveolar Epithelial Cells
Perforin
Surface Properties
Health
Cell Adhesion
Nucleic Acids
Cultured Cells
Spectrum Analysis
Epithelial Cells
Cholesterol
Lung
Membranes
Proteins
streptolysin O

ASJC Scopus subject areas

  • Biotechnology

Cite this

Biologically inspired sensing : Infrared spectroscopic analysis of cell responses to an inhalation health hazard. / Riley, Mark; DeRosa, Diana; Blaine, Jeanette; Potter, B. G.; Lucas, Pierre; Le Coq, David; Juncker, Christophe; Boesewetter, Dianne E.; Collier, Jayne M.; Boussard-Plédel, Catherine; Bureau, Bruno.

In: Biotechnology Progress, Vol. 22, No. 1, 01.01.2006, p. 24-31.

Research output: Contribution to journalArticle

Riley, M, DeRosa, D, Blaine, J, Potter, BG, Lucas, P, Le Coq, D, Juncker, C, Boesewetter, DE, Collier, JM, Boussard-Plédel, C & Bureau, B 2006, 'Biologically inspired sensing: Infrared spectroscopic analysis of cell responses to an inhalation health hazard', Biotechnology Progress, vol. 22, no. 1, pp. 24-31. https://doi.org/10.1021/bp050125d
Riley, Mark ; DeRosa, Diana ; Blaine, Jeanette ; Potter, B. G. ; Lucas, Pierre ; Le Coq, David ; Juncker, Christophe ; Boesewetter, Dianne E. ; Collier, Jayne M. ; Boussard-Plédel, Catherine ; Bureau, Bruno. / Biologically inspired sensing : Infrared spectroscopic analysis of cell responses to an inhalation health hazard. In: Biotechnology Progress. 2006 ; Vol. 22, No. 1. pp. 24-31.
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