Biological functions of biotinylated histones

Nagarama Kothapalli, Gabriela Camporeale, Alice Kueh, Yap C. Chew, Anna M. Oommen, Jacob B. Griffin, Janos Zempleni

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Histones H1, H2A, H2B, H3 and H4 are DNA-binding proteins that mediate the folding of DNA into chromatin. Various posttranslational modifications of histones regulate processes such as transcription, replication and repair of DNA. Recently, a novel posttranslational modification has been identified: covalent binding of the vitamin biotin to lysine residues in histones, mediated by biotinidase and holocarboxylase synthetase. Here we describe a novel peptide-based technique, which was used to identify eight distinct biotinylation sites in histones H2A, H3 and H4. Biotinylation site-specific antibodies were generated to investigate biological functions of histone biotinylation. Evidence was provided that biotinylation of histones plays a role in cell proliferation, gene silencing and cellular response to DNA damage.

Original languageEnglish (US)
Pages (from-to)446-448
Number of pages3
JournalJournal of Nutritional Biochemistry
Volume16
Issue number7
DOIs
StatePublished - Jul 1 2005

Fingerprint

Histones
Biotinylation
Post Translational Protein Processing
DNA
Biotinidase
DNA-Binding Proteins
Cell proliferation
Transcription
Biotin
Vitamins
Protein Folding
Gene Silencing
Lysine
Chromatin
DNA Replication
DNA Repair
Repair
DNA Damage
Genes
Cell Proliferation

Keywords

  • Biotin
  • Chromatin
  • DNA damage
  • Epigenetics
  • Histones
  • Human

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Nutrition and Dietetics
  • Clinical Biochemistry

Cite this

Kothapalli, N., Camporeale, G., Kueh, A., Chew, Y. C., Oommen, A. M., Griffin, J. B., & Zempleni, J. (2005). Biological functions of biotinylated histones. Journal of Nutritional Biochemistry, 16(7), 446-448. https://doi.org/10.1016/j.jnutbio.2005.03.025

Biological functions of biotinylated histones. / Kothapalli, Nagarama; Camporeale, Gabriela; Kueh, Alice; Chew, Yap C.; Oommen, Anna M.; Griffin, Jacob B.; Zempleni, Janos.

In: Journal of Nutritional Biochemistry, Vol. 16, No. 7, 01.07.2005, p. 446-448.

Research output: Contribution to journalArticle

Kothapalli, N, Camporeale, G, Kueh, A, Chew, YC, Oommen, AM, Griffin, JB & Zempleni, J 2005, 'Biological functions of biotinylated histones', Journal of Nutritional Biochemistry, vol. 16, no. 7, pp. 446-448. https://doi.org/10.1016/j.jnutbio.2005.03.025
Kothapalli N, Camporeale G, Kueh A, Chew YC, Oommen AM, Griffin JB et al. Biological functions of biotinylated histones. Journal of Nutritional Biochemistry. 2005 Jul 1;16(7):446-448. https://doi.org/10.1016/j.jnutbio.2005.03.025
Kothapalli, Nagarama ; Camporeale, Gabriela ; Kueh, Alice ; Chew, Yap C. ; Oommen, Anna M. ; Griffin, Jacob B. ; Zempleni, Janos. / Biological functions of biotinylated histones. In: Journal of Nutritional Biochemistry. 2005 ; Vol. 16, No. 7. pp. 446-448.
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