Biological activities of extracellular vesicles and their cargos from bovine and human milk in humans and implications for infants

Janos Zempleni, Ana Aguilar-Lozano, Mahrou Sadri, Sonal Sukreet, Sonia Manca, Di Wu, Fang Zhou, Ezra Mutai

Research output: Contribution to journalArticle

41 Citations (Scopus)

Abstract

Extracellular vesicles (EVs) in milk harbor a variety of compounds, including lipids, proteins, noncoding RNAs, and mRNAs. Among the various classes of EVs, exosomes are of particular interest, because cargo sorting in exosomes is a regulated, nonrandom process and exosomes play essential roles in cell-to-cell communication. Encapsulation in exosomes confers protection against enzymatic and nonenzymatic degradation of cargos and provides a pathway for cellular uptake of cargos by endocytosis of exosomes. Compelling evidence suggests that exosomes in bovine milk are transported by intestinal cells, vascular endothelial cells, and macrophages in human and rodent cell cultures, and bovine-milk exosomes are delivered to peripheral tissues in mice. Evidence also suggests that cargos in bovine-milk exosomes, in particular RNAs, are delivered to circulating immune cells in humans. Some microRNAs and mRNAs in bovine-milk exosomes may regulate the expression of human genes and be translated into protein, respectively. Some exosome cargos are quantitatively minor in the diet compared with endogenous synthesis. However, noncanonical pathways have been identified through which low concentrations of dietary microRNAs may alter gene expression, such as the accumulation of exosomes in the immune cell microenvironment and the binding of microRNAs to Toll-like receptors. Phenotypes observed in infantfeeding studies include higher Mental Developmental Index, Psychomotor Development Index, and Preschool Language Scale-3 scores in breastfed infants than in those fed various formulas. In mice, supplementation with plant-derived MIR- 2911 improved the antiviral response compared with controls. Porcine-milk exosomes promote the proliferation of intestinal cells in mice. This article discusses the above-mentioned advances in research concerning milk exosomes and their cargos in human nutrition. Implications for infant nutrition are emphasized, where permitted, but data in infants are limited.

Original languageEnglish (US)
Pages (from-to)3-10
Number of pages8
JournalJournal of Nutrition
Volume147
Issue number1
DOIs
StatePublished - Jan 1 2017

Fingerprint

Exosomes
Human Milk
Milk
MicroRNAs
Extracellular Vesicles
Cellular Microenvironment
Gene Expression
Untranslated RNA
Messenger RNA
Toll-Like Receptors
Endocytosis
Cell Communication
Antiviral Agents

Keywords

  • Biological activity
  • Bovine milk
  • Exosomes
  • Extracellular vesicles
  • Human milk
  • Infants
  • MicroRNA
  • Noncoding RNA

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Biological activities of extracellular vesicles and their cargos from bovine and human milk in humans and implications for infants. / Zempleni, Janos; Aguilar-Lozano, Ana; Sadri, Mahrou; Sukreet, Sonal; Manca, Sonia; Wu, Di; Zhou, Fang; Mutai, Ezra.

In: Journal of Nutrition, Vol. 147, No. 1, 01.01.2017, p. 3-10.

Research output: Contribution to journalArticle

Zempleni, Janos ; Aguilar-Lozano, Ana ; Sadri, Mahrou ; Sukreet, Sonal ; Manca, Sonia ; Wu, Di ; Zhou, Fang ; Mutai, Ezra. / Biological activities of extracellular vesicles and their cargos from bovine and human milk in humans and implications for infants. In: Journal of Nutrition. 2017 ; Vol. 147, No. 1. pp. 3-10.
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