Biointeraction analysis of immobilized antibodies and related agents by high-performance immunoaffinity chromatography

Erika Pfaunmiller, Annette C. Moser, David S. Hage

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

A method is described based on high-performance immunoaffinity chromatography for examining the interactions of immobilized antibodies or related binding agents with their targets. It is shown how this method can be used to obtain information on the binding, elution and regeneration kinetics of immobilized binding agents, such as those used with immunoaffinity supports. The theory behind this approach is briefly described and it is demonstrated how both the kinetic and thermodynamic properties of a biointeraction can be determined experimentally through this method. Several applications are used to illustrate this technique, including antibody-antigen interactions and the binding of aptamers with their targets in the presence of silica-based supports. The same approach can be adapted for use with other types of targets, binding agents and support materials.

Original languageEnglish (US)
Pages (from-to)130-135
Number of pages6
JournalMethods
Volume56
Issue number2
DOIs
StatePublished - Feb 1 2012

Fingerprint

Immobilized Antibodies
Chromatography
Kinetics
Thermodynamics
Silicon Dioxide
Regeneration
Thermodynamic properties
Antigens
Antibodies

Keywords

  • Antibody-antigen interactions
  • Aptamers
  • Biointeraction analysis
  • High-performance immunoaffinity chromatography
  • Immunoaffinity chromatography

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Biointeraction analysis of immobilized antibodies and related agents by high-performance immunoaffinity chromatography. / Pfaunmiller, Erika; Moser, Annette C.; Hage, David S.

In: Methods, Vol. 56, No. 2, 01.02.2012, p. 130-135.

Research output: Contribution to journalArticle

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