Bioinformatics-enabled identification of the HrpL regulon and type III secretion system effector proteins of Pseudomonas syringae pv. phaseolicola 1448A

Monica Vencato, Fang Tian, James R. Alfano, C. Robin Buell, Samuel Cartinhour, Genevieve A. DeClerck, David S. Guttman, John Stavrinides, Vinita Joardar, Magdalen Lindeberg, Philip A. Bronstein, John W. Mansfield, Christopher R. Myers, Alan Collmer, David J. Schneider

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

The ability of Pseudomonas syringae pv. phaseolicola to cause halo blight of bean is dependent on its ability to translocate effector proteins into host cells via the hypersensitive response and pathogenicity (Hrp) type III secretion system (T3SS). To identify genes encoding type III effectors and other potential virulence factors that are regulated by the HrpL alternative sigma factor, we used a hidden Markov model, weight matrix model, and type III targeting-associated patterns to search the genome of P. syringae pv. phaseolicola 1448A, which recently was sequenced to completion. We identified 44 high-probability putative Hrp promoters upstream of genes encoding the core T3SS machinery, 27 candidate effectors and related T3SS substrates, and 10 factors unrelated to the Hrp system. The expression of 13 of these candidate HrpL regulon genes was analyzed by real-time polymerase chain reaction, and all were found to be upregulated by HrpL. Six of the candidate type III effectors were assayed for T3SS-dependent translocation into plant cells using the Bordetella pertussis calmodulin-dependent adenylate cyclase (Cya) translocation reporter, and all were translocated. PSPPH1855 (ApbE-family protein) and PSPPH3759 (alcohol dehydrogenase) have no apparent T3SS-related function; however, they do have homologs in the model strain P. syringae pv. tomato DC3000 (PSPTO2105 and PSPTO0834, respectively) that are similarly upregulatcd by HrpL. Mutations were constructed in the DC3000 homologs and found to reduce bacterial growth in host Arabidopsis leaves. These results establish the utility of the bioinformatic or candidate gene approach to identifying effectors and other genes relevant to pathogenesis in P. syringae genomes.

Original languageEnglish (US)
Pages (from-to)1193-1206
Number of pages14
JournalMolecular Plant-Microbe Interactions
Volume19
Issue number11
DOIs
StatePublished - Nov 1 2006

Fingerprint

Pseudomonas syringae pv. phaseolicola
Pseudomonas syringae
type III secretion system
Regulon
regulon
Bioinformatics
Computational Biology
bioinformatics
Genes
hypersensitive response
Virulence
Gene encoding
Proteins
pathogenicity
proteins
genes
Genome
Sigma Factor
Bordetella pertussis
Alcohol Dehydrogenase

Keywords

  • Avr/Hop proteins

ASJC Scopus subject areas

  • Physiology
  • Agronomy and Crop Science

Cite this

Bioinformatics-enabled identification of the HrpL regulon and type III secretion system effector proteins of Pseudomonas syringae pv. phaseolicola 1448A. / Vencato, Monica; Tian, Fang; Alfano, James R.; Buell, C. Robin; Cartinhour, Samuel; DeClerck, Genevieve A.; Guttman, David S.; Stavrinides, John; Joardar, Vinita; Lindeberg, Magdalen; Bronstein, Philip A.; Mansfield, John W.; Myers, Christopher R.; Collmer, Alan; Schneider, David J.

In: Molecular Plant-Microbe Interactions, Vol. 19, No. 11, 01.11.2006, p. 1193-1206.

Research output: Contribution to journalArticle

Vencato, M, Tian, F, Alfano, JR, Buell, CR, Cartinhour, S, DeClerck, GA, Guttman, DS, Stavrinides, J, Joardar, V, Lindeberg, M, Bronstein, PA, Mansfield, JW, Myers, CR, Collmer, A & Schneider, DJ 2006, 'Bioinformatics-enabled identification of the HrpL regulon and type III secretion system effector proteins of Pseudomonas syringae pv. phaseolicola 1448A', Molecular Plant-Microbe Interactions, vol. 19, no. 11, pp. 1193-1206. https://doi.org/10.1094/MPMI-19-1193
Vencato, Monica ; Tian, Fang ; Alfano, James R. ; Buell, C. Robin ; Cartinhour, Samuel ; DeClerck, Genevieve A. ; Guttman, David S. ; Stavrinides, John ; Joardar, Vinita ; Lindeberg, Magdalen ; Bronstein, Philip A. ; Mansfield, John W. ; Myers, Christopher R. ; Collmer, Alan ; Schneider, David J. / Bioinformatics-enabled identification of the HrpL regulon and type III secretion system effector proteins of Pseudomonas syringae pv. phaseolicola 1448A. In: Molecular Plant-Microbe Interactions. 2006 ; Vol. 19, No. 11. pp. 1193-1206.
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AU - Buell, C. Robin

AU - Cartinhour, Samuel

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AU - Stavrinides, John

AU - Joardar, Vinita

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