Biodegradation of fuel oxygenates by sol-gel immobilized bacteria Aquincola tertiaricarbonis L108

Angela Pannier, Claudia Oehm, Axel R. Fischer, Peter Werner, Ulrich Soltmann, Horst Böttcher

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The development of long-term storable biofilters containing bacteria which are capable to degrade recalcitrant environmental compounds like the fuel oxygenates methyl tert-butyl ether (MTBE) and ethyl tert-butyl ether (ETBE) is of special interest for the treatment of contaminated water. Fuel oxygenates have been proven to be more persistent to biodegradation than other gasoline components. Only a few microorganisms, e.g. Aquincola tertiaricarbonis L108, are capable to biodegrade these substances.In order to develop highly efficient and long-term stable biocomposite materials for bioremediation, the applicability of sol-gel matrices for the immobilization of A. tertiaricarbonis was tested. Two different sol-gel immobilization techniques were used: (A) the immobilization within ceramic-like shapes by using the freeze-gelation technique and (B) sol-gel coatings on an inert, porous material. For comparison, the widely used alginate-bead immobilization technique has been applied.The embedding of A. tertiaricarbonis within freeze-gelation composites (method A) proved to be not applicable because this strain was too sensitive regarding the applied freezing and drying conditions even though cell-protecting additives were added. In contrast, A. tertiaricarbonis immobilized on porous expanded clay pellets (method B) could be stored under humid atmosphere at least for a time of 8 months without a significant reduction of their metabolic activity.

Original languageEnglish (US)
Pages (from-to)291-296
Number of pages6
JournalEnzyme and Microbial Technology
Volume47
Issue number6
DOIs
StatePublished - Nov 8 2010

Fingerprint

Polymethyl Methacrylate
Biodegradation
Immobilization
Sol-gels
Bacteria
Gels
Gelation
Ethers
Biofilters
Bioremediation
Alginate
Freezing
Microorganisms
Gasoline
Environmental Biodegradation
Porous materials
Water Purification
Drying
Clay
Ceramics

Keywords

  • Alginate beads
  • Aquincola tertiaricarbonis
  • Expanded clay pellets
  • Freeze-gelation biocers
  • Methyl tert-butyl ether
  • Sol-gel immobilization

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biochemistry
  • Applied Microbiology and Biotechnology

Cite this

Biodegradation of fuel oxygenates by sol-gel immobilized bacteria Aquincola tertiaricarbonis L108. / Pannier, Angela; Oehm, Claudia; Fischer, Axel R.; Werner, Peter; Soltmann, Ulrich; Böttcher, Horst.

In: Enzyme and Microbial Technology, Vol. 47, No. 6, 08.11.2010, p. 291-296.

Research output: Contribution to journalArticle

Pannier, Angela ; Oehm, Claudia ; Fischer, Axel R. ; Werner, Peter ; Soltmann, Ulrich ; Böttcher, Horst. / Biodegradation of fuel oxygenates by sol-gel immobilized bacteria Aquincola tertiaricarbonis L108. In: Enzyme and Microbial Technology. 2010 ; Vol. 47, No. 6. pp. 291-296.
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