Biochemical and biologic characterization of exosomes and microvesicles as facilitators of HIV-1 infection in macrophages

Irena Kadiu, Prabagaran Narayanasamy, Prasanta K. Dash, Wei Zhang, Howard Eliot Gendelman

Research output: Contribution to journalArticle

108 Citations (Scopus)

Abstract

Exosomes and microvesicles (MV) are cell membranous sacs originating from multivesicular bodies and plasma membranes that facilitate long-distance intercellular communications. Their functional biology, however, remains incompletely understood. Macrophage exosomes and MV isolated by immunoaffinity and sucrose cushion centrifugation were characterized by morphologic, biochemical, and molecular assays. Lipidomic, proteomic, and cell biologic approaches uncovered novel processes by which exosomes and MV facilitate HIV-1 infection and dissemination. HIV-1 was "entrapped" in exosome aggregates. Robust HIV-1 replication followed infection with exosome-enhanced fractions isolated from infected cell supernatants. MV- and exosome-facilitated viral infections are affected by a range of cell surface receptors and adhesion proteins. HIV-1 containing exosomes readily completed its life cycle in human monocyte-derived macrophages but not in CD4- cells. The data support a significant role for exosomes as facilitators of viral infection.

Original languageEnglish (US)
Pages (from-to)744-754
Number of pages11
JournalJournal of Immunology
Volume189
Issue number2
DOIs
StatePublished - Jul 15 2012

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Exosomes
HIV Infections
HIV-1
Macrophages
Virus Diseases
Multivesicular Bodies
Cell Surface Receptors
Life Cycle Stages
Centrifugation
Proteomics
Sucrose
Cell Membrane

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Biochemical and biologic characterization of exosomes and microvesicles as facilitators of HIV-1 infection in macrophages. / Kadiu, Irena; Narayanasamy, Prabagaran; Dash, Prasanta K.; Zhang, Wei; Gendelman, Howard Eliot.

In: Journal of Immunology, Vol. 189, No. 2, 15.07.2012, p. 744-754.

Research output: Contribution to journalArticle

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