Binding of actinomycin D to single-stranded DNA

Elizabeth A. Jares-Erijman, Reinhard Klement, Reinhard Machinek, Randy M. Wadkins, Besik I. Kankia, Luis A. Marky, Thomas M. Jovin

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The sequence specificity and structural aspects of the mode of interaction of the antitumor drug actinomycin D (AMD) with single-stranded DNA were studied by fluorescence, absorption and NMR spectroscopy, calorimetry, ultrasonic velocity and density measurements, and molecular modeling. The binding is length and sequence dependent, with the tetranucleotide motif TAGT showing the highest affinity. A 'hemi- intercalation' model for the interaction is proposed.

Original languageEnglish (US)
Pages (from-to)661-667
Number of pages7
JournalNucleosides and Nucleotides
Volume16
Issue number5-6
DOIs
StatePublished - Jan 1 1997

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Ultrasonic velocity measurement
Calorimetry
Molecular modeling
Single-Stranded DNA
Fluorescence Spectrometry
Fluorescence spectroscopy
Dactinomycin
Intercalation
Absorption spectroscopy
Ultrasonics
Antineoplastic Agents
Nuclear magnetic resonance spectroscopy
Magnetic Resonance Spectroscopy

ASJC Scopus subject areas

  • Biochemistry
  • Genetics

Cite this

Jares-Erijman, E. A., Klement, R., Machinek, R., Wadkins, R. M., Kankia, B. I., Marky, L. A., & Jovin, T. M. (1997). Binding of actinomycin D to single-stranded DNA. Nucleosides and Nucleotides, 16(5-6), 661-667. https://doi.org/10.1080/07328319708002931

Binding of actinomycin D to single-stranded DNA. / Jares-Erijman, Elizabeth A.; Klement, Reinhard; Machinek, Reinhard; Wadkins, Randy M.; Kankia, Besik I.; Marky, Luis A.; Jovin, Thomas M.

In: Nucleosides and Nucleotides, Vol. 16, No. 5-6, 01.01.1997, p. 661-667.

Research output: Contribution to journalArticle

Jares-Erijman, EA, Klement, R, Machinek, R, Wadkins, RM, Kankia, BI, Marky, LA & Jovin, TM 1997, 'Binding of actinomycin D to single-stranded DNA', Nucleosides and Nucleotides, vol. 16, no. 5-6, pp. 661-667. https://doi.org/10.1080/07328319708002931
Jares-Erijman EA, Klement R, Machinek R, Wadkins RM, Kankia BI, Marky LA et al. Binding of actinomycin D to single-stranded DNA. Nucleosides and Nucleotides. 1997 Jan 1;16(5-6):661-667. https://doi.org/10.1080/07328319708002931
Jares-Erijman, Elizabeth A. ; Klement, Reinhard ; Machinek, Reinhard ; Wadkins, Randy M. ; Kankia, Besik I. ; Marky, Luis A. ; Jovin, Thomas M. / Binding of actinomycin D to single-stranded DNA. In: Nucleosides and Nucleotides. 1997 ; Vol. 16, No. 5-6. pp. 661-667.
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