Biliary excretion of biotin and biotin metabolites is quantitatively minor in rats and pigs

Janos Zempleni, Gary M. Green, Alan W. Spannagel, Donald M. Mock

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

We sought to determine whether the biliary excretion of biotin contributes substantially to the overall excretion of the vitamin in mammals, and hence, whether metabolism by gut microorganisms could account for some metabolism of biotin administered parenterally, [carbonyl-14C]Biotin was injected intravenously into six rats; bile and urine were collected for 24 h after injection. In a study of five pigs, serum and bile were analyzed for endogenous biotin and metabolites. In rat bile and urine, biotin, bisnorbiotin, biotin-d,l-sulfoxide, bisnorbiotin methyl ketone and two unidentified compounds were quantitated. In bile, these six compounds accounted for only 1.9 ± 0.2% of the administered 14C, but in urine they accounted for 60.6 ± 4.1%. The metabolite and time profiles in bile were also strikingly different from those in urine. Only biotin, bisnorbiotin and biotin-d,l-sulfoxide were quantitated in pig bile and serum. The concentrations of biotin, bisnorbiotin and biotin-d,l-sulfoxide in bile were 6.9-14.7 times the concentrations in serum. However, the bile to serum ratios of biotin and metabolites were >99% less than those of bilirubin, which is actively excreted. These data provide evidence that the biliary excretion of biotin and metabolites is quantitatively negligible.

Original languageEnglish (US)
Pages (from-to)1496-1500
Number of pages5
JournalJournal of Nutrition
Volume127
Issue number8
StatePublished - Aug 14 1997

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biotin
Biotin
Swine
excretion
metabolites
bile
Bile
sulfoxide
swine
rats
urine
Urine
Serum
Hepatobiliary Elimination
metabolism
bilirubin
ketones
Bilirubin
Vitamins
vitamins

Keywords

  • Bile
  • Biotin
  • Pigs
  • Rats

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Biliary excretion of biotin and biotin metabolites is quantitatively minor in rats and pigs. / Zempleni, Janos; Green, Gary M.; Spannagel, Alan W.; Mock, Donald M.

In: Journal of Nutrition, Vol. 127, No. 8, 14.08.1997, p. 1496-1500.

Research output: Contribution to journalArticle

Zempleni, Janos ; Green, Gary M. ; Spannagel, Alan W. ; Mock, Donald M. / Biliary excretion of biotin and biotin metabolites is quantitatively minor in rats and pigs. In: Journal of Nutrition. 1997 ; Vol. 127, No. 8. pp. 1496-1500.
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