Bighorn sheep fetal lung cell line for detection of respiratory viruses

Sudarvili Shanthalingam, Christina Topliff, Clayton L Kelling, Subramaniam Srikumaran

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Pneumonia is an important disease of bighorn sheep (BHS) that is primarily responsible for the drastic decline in numbers of these animals in North America. Members of the genus Mannheimia and Pasteurella have frequently been isolated from the pneumonic lungs of BHS. Antibodies to several respiratory viruses, including bovine parainfluenza virus 3 (BPIV-3), bovine respiratory syncytial virus (BRSV), bovine viral diarrhea virus (BVDV), and bovine herpesvirus 1 (BoHV-1), have been detected in herds of BHS. The availability of BHS fetal lung cell lines is likely to enhance the chances of isolation of these viruses. Here we report the development of such a cell line. This line is permissive for BPIV-3, BRSV, BVDV, and BoHV-1 infection, as revealed by an enzyme immunoassay of virus-infected cells with antibodies specific for each of these viruses. This cell line should be valuable for detecting these 4, and possibly other, respiratory viruses in BHS.

Original languageEnglish (US)
Pages (from-to)75-77
Number of pages3
JournalCanadian Journal of Veterinary Research
Volume74
Issue number1
StatePublished - Jan 1 2010

Fingerprint

Bighorn Sheep
Ovis canadensis
lungs
cell lines
Bovine parainfluenza virus 3
Viruses
Cell Line
Lung
viruses
Bovine respiratory syncytial virus
Bovine Herpesvirus 1
Bovine Viral Diarrhea Viruses
Bovine herpesvirus 1
Bovine viral diarrhea virus
Mannheimia
Pasteurella
Herpesviridae Infections
antibodies
Antibodies
enzyme immunoassays

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Bighorn sheep fetal lung cell line for detection of respiratory viruses. / Shanthalingam, Sudarvili; Topliff, Christina; Kelling, Clayton L; Srikumaran, Subramaniam.

In: Canadian Journal of Veterinary Research, Vol. 74, No. 1, 01.01.2010, p. 75-77.

Research output: Contribution to journalArticle

Shanthalingam, S, Topliff, C, Kelling, CL & Srikumaran, S 2010, 'Bighorn sheep fetal lung cell line for detection of respiratory viruses', Canadian Journal of Veterinary Research, vol. 74, no. 1, pp. 75-77.
Shanthalingam, Sudarvili ; Topliff, Christina ; Kelling, Clayton L ; Srikumaran, Subramaniam. / Bighorn sheep fetal lung cell line for detection of respiratory viruses. In: Canadian Journal of Veterinary Research. 2010 ; Vol. 74, No. 1. pp. 75-77.
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